Results: 2-Step Fusion PCR Generates S. mutans Strains with Gene Disruptions
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Conclusion
Transcripción
The overall goal of this procedure is generate targeted gene-disrupted Streptococcus mutans strains using a two-step fusion PCR and electroporation.This method can help answer key questions in the microbiology field such as how a gene of interest
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We describe a facile, rapid, and relatively inexpensive method for the generation of gene-disrupted Streptococcus mutans strains; this technique may be adapted for the generation of gene-disrupted strains of various species.