Preparation of the Columns and Solutions Needed for the Extraction and Determination of Catecholamines
4:44
Preparation of Real Urine Samples and Mobile Phase
6:25
PFSPE Extraction and HPLC Analysis
9:58
Results: Identification and Quantification of Extracted Catecholamines and their Metabolites in Urine
11:43
Conclusion
Transcripción
The overall goal of this procedure is to determine three monoamine neurotransmitters and two of their metabolites in infant's urine simultaneously via a convenient solid-phase extraction coupled to high-pressure liquid chromatography with electroc
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We present a convenient solid-phase extraction coupled to high-pressure liquid chromatography (HPLC) with electrochemical detection (ECD) for simultaneous determination of three monoamine neurotransmitters and two of their metabolites in infants' urine. We also identify the metabolite MHPG as a potential biomarker for the early diagnosis of brain damage for infants.