The overall goal of this procedure is to generate a genetic mosaic in mouse cerebral cortex by inducing Cre-Lox recombination through in utero electroporation. This method of generating genetic mosaics can help answer key questions in neurobiology
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Cell-autonomous functions of genes in the brain can be studied by inducing loss or gain of function in sparse populations of cells. Here, we describe in utero electroporation to deliver Cre recombinase into sparse populations of developing cortical neurons with floxed genes to cause loss of function in vivo.