Results: Transient Protein Expression Enables Analysis of Anti-apoptotic Activity
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Conclusion
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The overall goal of this heat shock-induced protein expression system is to evaluate protein anti-apoptotic activity in two potentially functionally antagonistic proteins in insect cells. This method can help answer key questions about the functio
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This protocol describes a heat shock-induced protein expression system (pDHsp/V5-His/sf9 cell system), which can be used for either expressing foreign proteins or evaluating the anti-apoptotic activity of potential foreign proteins and their truncated amino acids in insect cells.