Artificial Wound Image Analysis and Migration Quantification
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Artificial Migration Front Assay
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Results: Representative Artificial Wound Scratch and Migration Front Assay Imaging
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Conclusion
Transcribir
The overall goal of these epithelial cell culture artificial wounding procedures is to study cell migration from both a quantitative and qualitive perspective, allowing assessment of the effects of specific treatments of interest on wound healing.
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This manuscript describes how incision-like lesions made on cultured epithelial cell monolayers conveniently model wound healing in vitro, allowing for imaging by confocal or laser scanning microscopy, and which can provide high-quality quantitative and qualitative data for studying both cell behavior and the mechanisms involved in migration.