Protocolo de diferenciación cerebelosa 3D optimizada con modificación 2D opcional

The overall goal of these differentiation cultures is to generate cerebellar cell lineages in 2D or 3D environments.These methods can help to answer basic questions regarding new developments or disease mechanisms underlying childhood brain disorders using patient derived stem cells.The main advantage of this technique is that it has a simple startup and maintenance with few factors and that it can be used as a base for testing more complex neural protocols.Demonstrating the procedure will be Lisa a technician from my laboratory.To set up the differentiation with modified method G-method of passing of hPSC's transfer the required volume of neural maintenance medium to a sterile tube and supplement with 4 nanograms per milliliter FGF2 and ten micromolar rock inhibitor.Then, keep the medium tube at room temperature or in water bath at 37