Isolation of Quail Embryonic Region Containing the Presumptive Territory of Thymic and Parathyroids Rudiments
5:49
Organotypic Assay: In Vitro Culture of the Embryonic Region Containing the Presumptive Territory of Thymic and Parathyroids Rudiments
8:17
Chorioallantoic Membrane (CAM) Assay: Grafting of Cultured Explants onto CAM, Ectopic Organ Formation after 10 Days in CAM
10:59
Representative Results of the Two-Step Approach
13:08
Conclusion
Transcripción
The overall goal of this procedure is to provide a two-step approach to study the early and late stages of organogenesis by combining in vitro manipulation of embryonic tissues with in ovo grafting techniques using the avian model. In this method
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This article provides an updated approach to the classical quail-chicken chimera system to study organ formation, by combining novel in vitro and in ovo experimental procedures.