Physical Alignment of Image Splitter Generated Images
2:25
Alignment of Spectrally Separated Channels by Software Performing Spatial Transformation
4:34
Single Molecule Imaging of Mitochondrial Membrane Proteins
8:32
Results: Multi-color Localization of Mammalian Mitochondrial PINK1
10:15
Conclusion
Transcripción
This technique can answer key questions in regulatory processes such as which role protein dynamics and organization play in functional adaptation. The main advantage of this technique is that it can be done in living cells, thereby revealing prot
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Here, we present a protocol for multi-color localization of single membrane proteins in organelles of live cells. To attach fluorophores, self-labeling proteins are used. Proteins, located in different membranes compartments of the same organelle, can be localized with a precision of ~18 nm.