Alignment of the Microscope Temporal Synchronization of the Beams Use of the Autocorrelator
3:24
Alignment of the Microscope Aligning the Femtosecond Laser Beams :OPO and Ti:Sa
4:44
Optimization of Temporal Synchronization of Beams
5:48
Optimization of Spatial Overlap of Beams - Transmission Image Acquisition of OPO and Ti:Sa
6:48
SRS Image Acquisition
7:40
Results: Nonlinear Microscope Based on Stimulated Raman Scattering
8:43
Conclusion
Transcripción
This protocol can help scientist interested in nonlinear microscopy to understand the key components, the setting up, and the alignment procedure of a microscope based on stimulated Raman scattering. The main advantages of SRS microscopy are its a
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In this manuscript, the implementation of a stimulated Raman scattering (SRS) microscope, obtained by the integration of an SRS experimental set-up with a laser scanning microscope, is described. The SRS microscope is based on two femtosecond (fs) laser sources, a Ti-Sapphire (Ti:Sa) and synchronized optical parametric oscillator (OPO).