September 28th, 2019
•Provided here is a protocol for investigating the interactions between native form, prefibrillar, and mature amyloid fibrils of different peptides and proteins with mitochondria isolated from different tissues and various areas of the brain.
Videos relacionados
Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro
Method to Visualize and Analyze Membrane Interacting Proteins by Transmission Electron Microscopy
A Tailored HPLC Purification Protocol That Yields High-purity Amyloid Beta 42 and Amyloid Beta 40 Peptides, Capable of Oligomer Formation
Imaging Amyloid Tissues Stained with Luminescent Conjugated Oligothiophenes by Hyperspectral Confocal Microscopy and Fluorescence Lifetime Imaging
Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag
Detection of Detergent-sensitive Interactions Between Membrane Proteins
Use of Two Dimensional Semi-denaturing Detergent Agarose Gel Electrophoresis to Confirm Size Heterogeneity of Amyloid or Amyloid-like Fibers
Generation of Native, Untagged Huntingtin Exon1 Monomer and Fibrils Using a SUMO Fusion Strategy
Detection of Small GTPase Prenylation and GTP Binding Using Membrane Fractionation and GTPase-linked Immunosorbent Assay
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados