Epithelial Progenitor Cell Enrichment from Tracheo-Bronchial Airways: Proximal Tissue Preparation
7:47
Proximal Lung Tissue Enzyme Digestion
8:51
Results: Representative Human Lung Epithelial Progenitor Cell Enrichment and Organoid Culture
10:48
Conclusion
Transcripción
3D organoid cultures are proximal in distal lung epithelium developed using this protocol. Provide tractable model to study fundamental mechanisms of lung tissue maintenance or disease associated remodeling, and serves an effective platform for dr
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This article provides a detailed methodology for tissue dissociation and cellular fractionation approaches allowing enrichment of viable epithelial cells from proximal and distal regions of the human lung. Herein these approaches are applied for the functional analysis of lung epithelial progenitor cells through the use of 3D organoids culture models.