Establishment of FM Cell Lines Lentvirus-hTERT Transduction
3:57
Lentivirus-MyoD Transduction
4:54
Fibroblasts Transdifferentiation
6:11
Results
9:16
Conclusion
Transcribir
Hello everyone. My name is Nicolas Wein. I'm a principal investigator at Nationwide Children's Hospital in Columbus, Ohio.
And today we're gonna show you a video about how to take a skin biopsy and convert it into myoblasts and myotube. This
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This protocol describes the conversion of skin fibroblasts into myoblasts and their differentiation into myotubes. The cell lines are derived from patients with neuromuscular disorders and can be used to investigate pathological mechanisms and to test therapeutic strategies.