This protocol will outline an easy and efficient way to successfully isolate primary mouse retinal pigmented epithelium cells. Retinal pigmented epithelium cells play a very important role to maintain the photoreceptor cells and to ensure the norm
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This manuscript describes a simplified protocol for the isolation of retinal pigmented epithelium (RPE) cells from mouse eyes in a stepwise manner. The protocol includes the enucleation and dissection of mouse eyes, followed by the isolation, seeding, and culturing of RPE cells.