Changing the Raman Shift and Collecting a Hyperspectral Data Stack
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Results: Hyperspectral Imaging of Gold Nanoparticles and Cancer Cells
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Conclusion
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Our protocol presents a multimodal, nonlinear, optical microscopy and applies it to perform chemically-specific imaging of gold nanoparticles in cancer cells. The main advantage of our imaging system is that it can be used to review the biomolecul
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This article presents the integration of a spectral-focusing module and a dual-output pulse laser, enabling rapid hyperspectral imaging of gold nanoparticles and cancer cells. This work aims to demonstrate the details of multimodal nonlinear optical techniques on a standard laser scanning microscope.