November 4th, 2022
•Presented here is a protocol for the induction and analysis of in vitro neutrophil extracellular traps (NETs). Quantification of DNA, cathelicidin (LL37), and enzyme activity yielded data that show the variability in the composition and morphology of NETs induced by microbial and chemical stimuli under similar controlled conditions.
Tags
Videos relacionados
Neutrophil Extracellular Traps: How to Generate and Visualize Them
Simplified Human Neutrophil Extracellular Traps (NETs) Isolation and Handling
Methods to Study Lipid Alterations in Neutrophils and the Subsequent Formation of Neutrophil Extracellular Traps
Visualizing Macrophage Extracellular Traps Using Confocal Microscopy
In Vitro Canine Neutrophil Extracellular Trap Formation: Dynamic and Quantitative Analysis by Fluorescence Microscopy
Automated Image-Based Quantification of Neutrophil Extracellular Traps Using NETQUANT
Identification of Neutrophil Extracellular Traps in Paraffin-Embedded Feline Arterial Thrombi using Immunofluorescence Microscopy
Quantifying Myeloperoxidase-DNA and Neutrophil Elastase-DNA Complexes from Neutrophil Extracellular Traps by Using a Modified Sandwich ELISA
Immunofluorescence Imaging of Neutrophil Extracellular Traps in Human and Mouse Tissues
Immunofluorescence Staining of Neutrophil Extracellular Traps in Tissue Samples
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados