The scope of our research focus on the precise quantitative diagnosis of thyroid disease, specifically exploring how different the status of the disease can be currently quantified. Currently, the fusion diagnosis of ultrasound imaging and the solid biochemical markers is being utilized to advance research in our field. We have established significant findings in our field by synchronizing B ultrasound and the color Doppler ultrasound in 3D space to diagnose solid imaging indicators and integrate them with biochemical indicators.

With our protocol, we are addressing the research gap by utilizing portable handheld ultrasound devices to achieve panoramic observation and diagnosis of the triplanar wheels of solid ultrasound imaging. Our protocol offers the advantage of high cost effectiveness and comprehensive precise diagnosis compared to other techniques. After acquiring a sequence of transverse B-mode images, visualizing thyroid morphology, copy all DICOM data to a customized working directory.

Open the MATLAB on the computer. In the command window, enter the indicated command to view the size of the size_vb0 data. Next, enter the indicated command to call the us_b show function to convert the four-dimensional matrix into a continuous gray scale video sequence.

Press the play button to initiate continuous video playback of the frame sequence. Use the pause and play control tool icons for flexible navigation of any frame. Use the zoom in or out buttons to dynamically magnify or minify the images during playback.

Set the default zoom button to the original one X view. For localized analysis, click on the inspect pixel values button and move the mouse over a region to overlay crosshairs with pixel coordinates and intensities. Using the DICOM read function, import the color Doppler ultrasound data of thyroid into MATLAB, then select the size function to view the dimensions of the data.

Next, use the us_c_show function to transform the 4D matrix data into a continuous color video sequence for detailed examination. After loading the B-mode and color Doppler four-dimensional ultrasound files of thyroid morphology, execute the synchronize_b_c function with the two four-dimensional matrices as inputs to view the dimensions of the data. Using the thyroid_triplanar function with the four-dimensional B-mode ultrasound data, generate three orthogonal planes of the thyroid gland.

Click and drag the center of the crosshair for an arbitrary 3D examination of the thyroid anatomy reconstructed from ultrasound. Press and drag the left mouse button over any region of the images for real-time modification of brightness and contrast levels. Release the mouse button to confirm and finalize the adjustments.

Using the thyroid_3D_blood function with the four-dimensional C-mode ultrasound data, generate three orthogonal planes of the thyroid glands vascular structure. Click and drag the center of the crosshair for an arbitrary 3D examination of the thyroid anatomy reconstructed from ultrasound. Press and drag the left mouse button over any region of the images for real-time modification of brightness and contrast levels.

Release the mouse button to confirm and finalize the adjustments. Drag the crosshair interaction and triplanar views extracted from B-Mode ultrasound to locate the region of interest. Execute the us_b2c to obtain the corresponding location in the color Doppler triplanar views.

Drag the crosshair interaction and triplanar views extracted from Doppler ultrasound to locate the region of interest. Then execute us_c2b to obtain the corresponding location in the B-mode triplaner views. The synchronized visualization of structural and functional information across three orthogonal planes significantly aided in the precise localization and severity determination of thyroid pathologies.