Temporal Regulation of Phosphatase Activity with the Rapamycin-Regulated System
September 6th 2024
Author Spotlight: Developing Tools to Tune the Activity of Tyrosine Phosphatases
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Preparing Cells Expressing Rapamycin-Regulated Phosphatase for Immunoprecipitation to Assess Temporal Control of Tyrosine Phosphatases
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Immunoprecipitation of RapR-Shp2 to Study Chemogenetic Control of Tyrosine Phosphatase
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Phosphatase Activity Assay to Characterize Allosteric Regulation of Phosphatases with the Rapamycin-Regulated RapR System
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This protocol describes the design, creation, and application of rapamycin-regulated phosphatases. This method provides high specificity and tight temporal control of phosphatase activation in living cells.
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