S'identifier

Guangzhou Darui Biotechnology Co., Ltd

2 ARTICLES PUBLISHED IN JoVE

Erratum: Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Kaihua Huang¹, Xiao Yan¹, Zhicheng Huang¹, Huayuan Liang¹, Zhiwei Li¹, Minghao Wang¹, Yong Wan², Suihai Wang^3,4, Liying Zhao¹

¹Department of General Surgery, Nanfang Hospital, The First School of Clinical Medicine, Southern Medical University, ²Research and Development Department, Guangzhou Darui Biotechnology Co., Ltd, ³Key Laboratory of Antibody Engineering of Guangdong Higher Education Institutes, School of Laboratory Medicine and Biotechnology, Southern Medical University, ⁴Provincial Key Laboratory of Immune Regulation and Immunotherapy, Southern Medical University

An erratum was issued for: Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform. The Authors section was updated.

Medicine

Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform

Kaihua Huang¹, Xiao Yan¹, Zhicheng Huang¹, Huayuan Liang¹, Zhiwei Li¹, Minghao Wang¹, Yong Wan², Suihai Wang^3,4, Liying Zhao¹

¹Nanfang Hospital, Southern Medical University, ²Research and Development Department, Guangzhou Darui Biotechnology Co., Ltd, ³Key Laboratory of Antibody Engineering of Guangdong Higher Education Institutes, School of Laboratory Medicine and Biotechnology, Southern Medical University, ⁴Provincial Key Laboratory of Immune Regulation and Immunotherapy, Southern Medical University

This protocol proposes holistic laboratory procedures required to detect single nucleotide polymorphisms in gastric cancer samples based on an ion semiconductor sequencing platform. The target sequences, ligating adapters, library amplification and purification, and quality control criteria are also described in detail.