Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing PlatformKaihua Huang 1, Xiao Yan 1, Zhicheng Huang 1, Huayuan Liang 1, Zhiwei Li 1, Minghao Wang 1, Yong Wan 2, Suihai Wang 3,4, Liying Zhao 1
1Nanfang Hospital, Southern Medical University, 2Research and Development Department, Guangzhou Darui Biotechnology Co., Ltd, 3Key Laboratory of Antibody Engineering of Guangdong Higher Education Institutes, School of Laboratory Medicine and Biotechnology, Southern Medical University, 4Provincial Key Laboratory of Immune Regulation and Immunotherapy, Southern Medical University
This protocol proposes holistic laboratory procedures required to detect single nucleotide polymorphisms in gastric cancer samples based on an ion semiconductor sequencing platform. The target sequences, ligating adapters, library amplification and purification, and quality control criteria are also described in detail.
