University of Malta

2 ARTICLES PUBLISHED IN JoVE

Bioengineering

Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival Material

Shawn Baldacchino¹, Christian Saliba², Jeanesse Scerri³, Christian Scerri³, Godfrey Grech¹

¹Department of Pathology, Faculty of Medicine & Surgery, University of Malta, ²Centre for Molecular Medicine and Biobanking, University of Malta, ³Department of Physiology & Biochemistry, Faculty of Medicine and Surgery, University of Malta

Nucleic acid degradation in archival tissue, tumor heterogeneity, and a lack of fresh frozen tissue specimens can negatively impact cancer diagnostic services in pathology laboratories worldwide. This manuscript describes the optimization of a panel of biomarkers using a multiplex magnetic bead assay to classify breast cancers.

Biology

Small Molecule Screening and Toxicity Testing in Early-stage Zebrafish Larvae

Margherita Muscat¹, Sherif Suleiman^2,3, Melissa M. Formosa^1,3

¹Department of Applied Biomedical Science, Faculty of Health Sciences, University of Malta, ²Department of Anatomy, Faculty of Medicine and Surgery, University of Malta, ³Centre for Molecular Medicine and Biobanking, University of Malta

This protocol describes a drug screening procedure in zebrafish larvae 3-7 days post fertilization followed by morphological assessment.