Optimization of a Multiplex RNA-based Expression Assay Using Breast Cancer Archival MaterialShawn Baldacchino 1, Christian Saliba 2, Jeanesse Scerri 3, Christian Scerri 3, Godfrey Grech 1
1Department of Pathology, Faculty of Medicine & Surgery, University of Malta, 2Centre for Molecular Medicine and Biobanking, University of Malta, 3Department of Physiology & Biochemistry, Faculty of Medicine and Surgery, University of Malta
Nucleic acid degradation in archival tissue, tumor heterogeneity, and a lack of fresh frozen tissue specimens can negatively impact cancer diagnostic services in pathology laboratories worldwide. This manuscript describes the optimization of a panel of biomarkers using a multiplex magnetic bead assay to classify breast cancers.
