Rapid (Vial-Based) Protein Precipitation with Acetone
2:27
Protein Precipitation by Chloroform/Methanol/Water (CMW)
4:30
Protein Precipitation Using a Disposable Filtration Cartridge
5:45
Resolubilization of Protein Pellet with Formic Acid
6:58
Solid-Phase Extraction (SPE) Clean-Up
8:20
Results: Protein Precipitation via Vial-Based Versus Cartridge Approach
10:22
Conclusion
Transcription
These protocols are designed to maximize the recovery as well as the purity of proteins through solvent-based precipitation. We're gonna show you a couple tricks to perform precipitation in vials, as well as a semi-automated approach in a spin car
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The present protocol describes solvent-based protein precipitation under controlled conditions for robust and rapid recovery and purification of proteome samples prior to mass spectrometry.