Cell Membrane Immobilization on IAM.PC.DD2 Particles
6:23
CMAC Column Packing
8:57
Results: Antibody Staining and Frontal Affinity Chromatography of Immobilized TrkB Receptors
11:02
Conclusion
Transcription
This vertical presents important steps in assembling cell membrane affinity chromatography columns with functional transmembrane receptors. These columns can be used for the identification of small molecules present in complex extracts and interac
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The protocol describes the preparation of cell membrane affinity chromatography (CMAC) columns with immobilized cell membrane fragments containing functional transmembrane tropomyosin kinase receptor B proteins. The use of CMAC columns in the identification of specialized plant metabolites interacting with these receptors and present in complex natural mixtures is also explained.