Name: creation of murine experimental abdominal aortic aneurysms with elastase
Uploaded: 2009-07-23T18:00:00
Description: Watch this Scientific Journal Video about Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase at JoVE.com

<p class="jove_content">This work was supported the SCCOR grant &ldquo;AAA Disease:  Mechanisms, Stratification, and Treatment&rdquo; (P50 HL083800-03) from the National Institutes of Health</p>

<ol>
<li>Mice are anesthetized with inhaled isoflurane 2.0-3.0ml/L.</li>
<li>The abdomen is shaved and scrubbed with alcohol and betadine.</li>
<li>The mouse is placed supine on the operative field.</li>
<li>Sterile gloves are worn by the surgeon, and the instruments used have been sterilized with hot bead sterilizer.</li>
<li>A long midline abdominal incision is made. Abdominal contents are retracted outside the abdomen with sterile gauze to expose the abdominal aorta.</li>
<li>The abdominal aorta is isolated from the level of the left renal v...

<ol>
	<li>Anidjar, S., Salzmann, J. L., Gentric, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Elastase-induced+experimental+aneurysms+in+rats.">Elastase-induced experimental aneurysms in rats.</a> <em style='font-style: italic'>Circulation</em>. <b>82</b> (3), 973-973 (1990).</li><li>Pyo, R., Lee, J. K., Shipley, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeted+gene+disruption+of+matrix+metalloproteinase-9+(gelatinase+B)+suppresses+development+of+experimental+abdominal+aortic+aneurysms.">Targeted gene disruption of matrix metalloproteinase-9 (gelatinase B) suppresses development of experimental abdominal aortic aneurysms.</a> <em style='font-style: italic'>J Clin Invest</em>. <b>105</b> (11), 1641-1641 (2000).</li><li>Carsten, C. G., Calton, W. C., Johanning, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Elastase+is+not+sufficient+to+induce+experimental+abdominal+aortic+aneurysms.">Elastase is not sufficient to induce experimental abdominal aortic aneurysms.</a> <em style='font-style: italic'>J Vasc Surg</em>. <b>33</b> (6), 1255-1255 (2001).</li></ol>

<p class="jove_content">Previous studies have noted significant lot-to-lot variation in AAA development from different commercial elastase preparations<sup>3</sup>. Thus, it is critical to monitor production lots as well as to have appropriate control groups in order to document the ability of the preparation to induce AAAs.</p>
<p class="jove_content">Interestingly, susceptibility to AAA development in this model is dependent on the mouse strain used<sup>3</sup>. The mouse used in the current video is of the C57Bl/6 strain which has consisten...

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<td>Type I porcine pancreatic elastase</td>
<td>Reagent</td>
<td>Sigma</td>
<td>E1250</td>
<td>&nbsp;</td>
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<td>polyethylene tubing (PE-10)</td>
<td>&nbsp;</td>
<td>CLAY ADAMS</td>
<td>427400</td>
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<td>CHANGE-A-TIP</td>
<td>&nbsp;</td>
<td>Bovie</td>
<td>18010-00 (FST)</td>
<td>cautery knife</td>
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<td>LEICA MZ6</td>
<td>&nbsp;</td>
<td>Leica</td>
<td>&nbsp;</td>
<td>stereomicroscope</td>
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<td>LEICA DFC290HD</td>
<td>&nbsp;</td>
<td>Leica</td>
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<td>USB camera</td>
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creation of murine experimental abdominal aortic aneurysms with elastase 

<p class="jove_content">Transient intraluminal infusion of porcine pancreatic elastase into the infrarenal segment of the abdominal aorta is the most widely used animal model of abdominal aortic aneurysm (AAA) ever since it was first described in rats by Anidjar and colleagues.<sup>1</sup> The rationale for its development was based on the disrupted nature of elastin observed in AAAs. This rat model has been modified to produce AAAs in the infrarenal aortic region of mice.<sup>2</sup> The model has the ability to add broad insight into the pathobiology of AAA due to the emergence of numerous transgenic and gene knockout mice.   Moreover, it is a viable platform to test potential therapeutic agents for AAA. In this video, we demonstrate the elastase infusion AAA procedure used in our laboratory.</p> 
<p class="jove_content">Mice are anesthetized using 2.5% isoflurane, and a laparotomy is performed under sterile conditions. The abdominal aortais isolated with the assistance of an operating stereomicroscope (Leica). After placing temporary ligatures around the proximal and distal aorta, an aortotomy is created at the bifurcation with the tip of a 30-gauge needle. A heat-tapered segment of PE-10 polyethylene tubing is introduced through the aortotomy and secured.  The aortic lumen is subsequently perfused for 5-15 minutes at 100 mm Hg with saline containing type I porcine pancreatic elastase (4.5 U/mL; Sigma Chemical Co.). After removing the perfusion catheter, the aortotomy is repaired without constriction of the lumen.</p>

Watch this Scientific Journal Video about Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase at JoVE.com

Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase

<p class="jove_content">This video shows how to induce abdominal aortic aneurysms (AAA) in mice via transient intraluminal infusion of porcine pancreatic elastase into the infrarenal segment of the abdominal aorta. The model has the ability to add broad insight into the pathobiology of AAA due to the emergence of numerous transgenic and gene knockout mice.</p>

Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase 

Transient intraluminal infusion of porcine pancreatic elastase into the infrarenal segment of the abdominal aorta is the most widely used animal model of ...

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