January 30th, 2012
•We present a method to visualize cuticle in live C. elegans using the red fluorescent lipophilic dye DiI (1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate), which is commonly used in C. elegans to visualize environmentally exposed neurons. With this optimized protocol, alae and annular cuticular structures are stained by DiI and observed using compound microscopy.
Vidéos Connexes
Measuring Caenorhabditis elegans Life Span in 96 Well Microtiter Plates
Basic Caenorhabditis elegans Methods: Synchronization and Observation
Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory
DiI Perfusion as a Method for Vascular Visualization in Ambystoma mexicanum
Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
Automating Aggregate Quantification in Caenorhabditis elegans
Hand Dissection of Caenorhabditis elegans Intestines
Isolation and Characterization of the Natural Microbiota of the Model Nematode Caenorhabditis elegans