Results: Consequences of Synaptic Activity Suppression on the Membrane Potential Dynamics and on Cortical Neurons Excitability
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Conclusion
Transcription
The overall goal of this protocol is to induce a general state of cerebral inactivity in vivo to study single neurons integrated properties in the absence of synaptic inputs. The method we present here can help to answer key questions in neurophys
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This procedure performs long-lasting in vivo intracellular recordings from single neurons during physiologically relevant cerebral states and after complete abolition of ongoing electrical activities, resulting in an isoelectric brain state. The physiological constants of the animal are carefully monitored during the transition to the artificial comatose condition.