Simultaneous Recordings of Ca2+ Transients and Contraction
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Analysis of the Myofilament Ca2+ Sensitivity
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Conclusion
Transcription
The overall goal of this procedure is to access the myofilament calcium sensitivity in order to enhance our understanding of the mechanisms that underlie the contraction in healthy and diseased hearts. This method can help answer to questions rela
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This paper describes a protocol that assesses the changes of myofilament Ca2+ sensitivity during contraction in isolated cardiac myocytes from rat heart. Together with cardiac electrophysiology, systolic/diastolic cytosol Ca2+ levels and contraction/relaxation, this measurement is imperative in underpinning the mechanisms mediating cardiac excitation-contraction coupling in healthy and diseased hearts.