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JoVE Journal

Neuroscience

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Preparation of Single-cohort Colonies and Hormone Treatment of Worker Honeybees to Analyze Physiology Associated with Role and/or Endocrine System

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08:53 min

September 6th, 2016

September 6th, 2016

15,695 Views

0:05

Title

0:36

Preparation of Single-cohort Colonies

2:28

Classifying Hypopharyngeal Glands (HPGs)

4:09

Injection of Active Ecdysone (20E)

5:24

Application of Methoprene, a JH Analogue

6:23

Results: mrjp2 and Hbg3 Expression in the HPG of Worker Honeybees

7:48

Conclusion

Transcription

The overall goal of these procedures is to examine the physiology of worker honeybees in association with the workers'role and the status of their endocrine system. It's a method which has been widely used for the analysis of worker behavior, can

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Here we describe our detailed protocol for the preparation of single-cohort honeybee colonies – a useful tool for analyzing the role-associated worker physiology. We also describe detailed protocols for treating workers with juvenile hormone and ecdysone to evaluate the involvement of these hormones in the regulation of worker behavior and/or physiology.

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