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JoVE Journal

Immunology and Infection

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Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

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06:46 min

June 30th, 2016

June 30th, 2016

28,477 Views

0:05

Title

0:50

Isolation and Cryopreservation of Monocytes

1:58

Differentiation of Monocytes into Macrophages

3:31

Harvesting Macrophages to Initiate Experiments

5:30

Results: Comparison of Fresh versus Cryopreserved Monocytes Used to Produce Macrophages

6:12

Conclusion

Transcription

The overall goal of this procedure is to improve the availability of monocytes and to minimize the variation in obtained cell densities during MCSF induced differentiation of human blood monocytes into macrophages. This method can help answer key

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A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. The protocol utilizes cryopreservation of monocytes coupled with their bulk differentiation into macrophages. Then harvested macrophages can then be seeded into culture wells at required cell densities for carrying out experiments.

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