Results: Oligonucleotide-directed Elution of RNA-protein Complexes
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Conclusion
Transcription
The overall goal of this RNA immunoprecipitation technique is to selectively isolate and characterize endogenous RNA protein complexes formed in eukaryotic cells via a specific oligonucleotide directed enrichment. This method can help answer key q
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This manuscript describes an approach to isolate select cognate RNPs formed in eukaryotic cells via a specific oligonucleotide-directed enrichment. We demonstrate the applicability of this approach by isolating a cognate RNP bound to the retroviral 5' untranslated region that is composed of DHX9/RNA helicase A.