Loading Yeast Cells into Prepared Flow Chamber Slides
5:03
Image Acquisition
6:30
Image Analysis
8:15
Results: Astral Microtubule Dynamics in Altered in tub2-430Δ Mutant Cells
9:32
Conclusion
Transcription
The overall goal of this procedure is to measure microtubule dynamics in yeast cells. This method can help answer key questions in the microtubule field, such as how mutations in tubules and microtubule regulators regulate microtubule dynamics in
Connectez-vous ou commencez votre essai gratuit pour accéder à ce contenu.
Budding yeast is an advantageous model for studying microtubule dynamics in vivo due to its powerful genetics and the simplicity of its microtubule cytoskeleton. The following protocol describes how to transform and culture yeast cells, acquire confocal microscopy images, and quantitatively analyze microtubule dynamics in living yeast cells.