Preparing the Horizontal Native Polyacrylamide Gel
2:27
Binding Reaction
3:23
Analysis of Samples
4:12
Imaging the Gel
5:04
Results: Horizontal Gel Electrophoresis Enhances the Detection of Distinct RNA-protein Complexes
6:08
Conclusion
Transcription
The overall of this protocol is to analyze RNA protein interactions using horizontal native gel electrophoresis. This method can help answer key questions in RNA biochemistry, such as defining the characteristics of specific RNA protein interactio
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Native polyacrylamide gel electrophoresis is a fundamental tool for analyzing RNA-protein interactions. Traditionally most experiments have used vertical gels. However, horizontal gels provide several advantages, such as the opportunity to monitor complexes during electrophoresis. We provide a detailed protocol for generating and using horizontal native gel electrophoresis.