Results: Representative Results from a Synaptosomal DA Uptake Experiment of Four C57BI/6 Wild Type Mice from Drd1a-cre Mouse Strain
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Conclusion
Transcription
We are introducing two techniques in this protocol. The goal of the first, is to assess dopamine transporter function by measuring dopamine uptake from synaptosomal preparations. The second, is for assessing levels of dopamine in tissue by HPLC an
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Synaptosomal dopamine uptake and high-performance liquid chromatography analysis represent experimental tools to investigate dopamine homeostasis in mice by assessing the function of the dopamine transporter and levels of dopamine in striatal tissue, respectively. Here we present protocols to measure dopamine tissue content and assess the functionality of the dopamine transporter.