Aorta-gonad-mesonephros (AGM) Explant Culture and Colony Forming Units CFU) Analysis |
4:24
In Vivo AGM Transplantation
6:08
Results: Representative Effects of Fluoxetine on Hematopoietic Stem Cell (HSC) Development
7:08
Conclusion
Transcription
The overall goal of this experiment is to use an aorta-gonad-mesonephros explant culture to investigate the critical regulators of hematopoietic stem cell development. This method can help answer key questions in HSC biology and function fields ab
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This protocol describes using cultured Aorta-Gonad-Mesonephros for expression analyses, colony-forming units in the culture and spleen, and long-term reconstitution to determine the effect of regulatory factors and signaling pathways on hematopoietic stem cell development. This has been demonstrated as an effective system for studying hematopoietic stem cell biology and function.