Design and Construct Tissue Microarrays (TMAs) Based on the Kononen Technique
6:20
Silver In Situ Hybridization (SISH) Analysis of Human Epidermal Growth Factor Receptor 2 (HER2)
9:11
Results: Analysis of Heterogeneity of HER2 Gene Amplification in Breast Cancers
10:00
Conclusion
Transcription
The overall goal of this laboratory procedure is to simultaneously define, quantify and compare HER2 intra-tumor genetic heterogeneity in a large series of heterogeneously processed breast cancers by means of high-throughput silver in situ hybridi
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Heterogeneous distribution of HER2-positive cells can be observed in a subset of breast cancers and generates clinical dilemmas. Here, we introduce a reliable and cost-effective protocol to define, quantify, and compare HER2 intra-tumor genetic heterogeneity in a large series of heterogeneously processed breast cancers.