Building Up a High-throughput Screening Platform to Assess the Heterogeneity of HER2 Gene Amplification in Breast Cancers

12.4K Views

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11:34 min

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December 5th, 2017

DOI :

10.3791/56686-v

December 5th, 2017

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Giulia Ercoli¹, Gianluca Lopez¹^,², Camilla Ciapponi¹^,³, Chiara Corti¹^,², Luca Despini⁴, Donatella Gambini⁵, Letterio Runza¹, Concetta Blundo⁴, Amedeo Sciarra¹, Nicola Fusco¹^,⁶

¹Division of Pathology, Fondazione IRCCS Ca' Granda, Ospedale Maggiore Policlinico, ²School of Medicine, University of Milan, ³School of Biology, University of Pavia, ⁴Division of Breast Surgery, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, ⁵Division of Medical Oncology, Fondazione IRCCS Ca' Granda - Ospedale Maggiore Policlinico, ⁶Department of Biomedical, Surgical, and Dental Sciences, University of Milan

Summary

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Keywords High throughput Screening

HER2 Gene Amplification

Breast Cancer

Silver In Situ Hybridization

Heterogeneity

Tissue Microarray

Archival Samples

Retrospective Studies

Fixation

Processing

Storage

Chapters in this video

0:05

Title

1:24

Design and Construct Tissue Microarrays (TMAs) Based on the Kononen Technique

6:20

Silver In Situ Hybridization (SISH) Analysis of Human Epidermal Growth Factor Receptor 2 (HER2)

9:11

Results: Analysis of Heterogeneity of HER2 Gene Amplification in Breast Cancers

10:00

Conclusion

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