Micropatterning Hydrocarbon-containing Compounds to Confine Fusion to Predetermined Regions
3:58
Fabricating Glass Bottom Dishes
4:55
Collecting Thioglycollate-elicited Macrophages
6:15
Results: Macrophage Fusion on Glass Surfaces
7:51
Conclusion
Transcription
The overall goal of this protocol is to transform non-fusogenic cover glass into a fusogenic surface that can be used to study macrophage fusion using any form of optical microscopy. This method can help to answer the fundamental questions related
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This protocol describes the fabrication of optical-quality glass surfaces adsorbed with compounds containing long-chain hydrocarbons that can be used to monitor macrophage fusion of living specimens and enables super-resolution microscopy of fixed specimens.