Soil Cell Lysis and Extraction of Metabolites, Proteins, and Lipids
7:55
Results: Analysis of the MPLEx Protocol for Extraction of Soil Samples
9:32
Conclusion
Transcription
This method is used in the proteomics, metabolomics, and lipidomics fields by helping obtain comprehensive information about the molecular changes taking place in soil-associated microbial communities due to environmental perturbations. The main a
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A protocol is presented for simultaneously extracting metabolites, proteins, and lipids from a single soil sample, allowing reduced sample preparation times and enabling multi-omic mass spectrometry analyses of samples with limited quantities.