S'identifier

JoVE Journal

Biochemistry

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Probing The Structure And Dynamics Of Nucleosomes Using Atomic Force Microscopy Imaging

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09:52 min

January 31st, 2019

January 31st, 2019

11,434 Views

0:04

Title

1:06

Functionalization of Mica Surface for Static AFM Imaging of Nucleosomes

2:37

Preparation of Nucleosome Samples on APS-Mica for Static AFM Imaging

3:39

Static AFM Imaging of Nucleosomes

4:42

High-Speed Time-Lapse AFM Imaging of Nucleosome Dynamics

7:42

Results: Probing the Structure and Dynamics of Nucleosomes

9:29

Conclusion

Transcription

This protocol utilizes all major features of AFM, non-amenable to other single molecule techniques. As a result, we are able to resolve structure of nucleosome at nano scale. Importantly, direct visualization of nucleosomes was done at physiologic

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Here, we present a protocol to characterize nucleosome particles at the single-molecule level using static and time-lapse atomic force microscopy (AFM) imaging techniques. The surface functionalization method described allows for the capture of the structure and dynamics of nucleosomes in high-resolution at the nanoscale.

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