Debris Removal and Crude Mitochondrial and Membrane Fraction Isolation
4:47
Isopycnic Density Gradient Purification
6:34
Nuclear Protein Isolation
7:57
Results: Fractionation of U937 Cells by Isopycnic Density Gradient Purification
9:34
Conclusion
Transcription
This protocol is significant, because it provides a method to separate four cellular compartments from one another, the cytoplasm, the nucleus, the mitochondria, and the membrane. Not only that, but it's a scalable, reproducible procedure that has
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This fractionation protocol will allow researchers to isolate cytoplasmic, nuclear, mitochondrial, and membrane proteins from mammalian cells. The latter two subcellular fractions are further purified via isopycnic density gradient.