We are thankful to the ACS PRF (grant 60302-UR9), Agrobio S.L. (contract #311325), and MCIN/AEI/10.13039/501100011033/FEDER, UE (grant No. PID2021-122369NB-I00).

1. Making simple drops
<ol>
	<li>For making simple drops, use a glass base made with a microscope slide (76.2 mm x 25.4 mm) to build the device. This allows for easy transportation and visualization of the liquids through the glass.</li>
	<li>Use a round glass capillary for the tip. For this protocol, use 1 mm diameter round capillaries (readily available in a wide range of sizes).
	<ol>
		<li>To make a tip with the desired diameter, pull the capillary using a micropipette pulling machine until two hal...

<ol>
	<li>Basaran, O. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Small-scale+free+surface+flows+with+break-up:+drop+formation+and+emerging+applications.">Small-scale free surface flows with break-up: drop formation and emerging applications.</a> American Institute of Chemical Engineers. 48 (9), 1842-1848 (2004).</li><li>Squires, T. M., Quake, S. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidics:+Fluid+physics+at+the+nanoliter+scale.">Microfluidics: Fluid physics at the nanoliter scale.</a> Reviews of Modern Physics. 77 (3), 977-1026 (2005).</li><li>Stone, H. A., Stroock, A. D., Adjari, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Engineering+Flows+in+Small+Devices:+Microfluidics+Toward+a+Lab-on-a-Chip.">Engineering Flows in Small Devices: Microfluidics Toward a Lab-on-a-Chip.</a> Annual Review of Fluid Mechanics. 36 (1), 381-411 (2004).</li><li>Gunther, A., Jensen, K. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multiphase+microfluidics:+from+flow+characteristics+to+chemical+and+materials+synthesis.">Multiphase microfluidics: from flow characteristics to chemical and materials synthesis.</a> Lab on a Chip. 6, 1487-1503 (2006).</li><li>Barrero, A., Loscertales, I. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Micro-+and+Nanoparticles+via+Capillary+Flows.">Micro- and Nanoparticles via Capillary Flows.</a> Annual Review of Fluid Mechanics. 39, 89-106 (2007).</li><li>Clift, R., Grace, J. R., Weber, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Bubbles, Drops, and Particles. , (2005).</li><li>Othmer, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Encyclopedia of Chemical Technology. 4th edition. 9, (1994).</li><li>Kentish, S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+use+of+ultrasonics+for+nanoemulsion+preparation.">The use of ultrasonics for nanoemulsion preparation.</a> Innovative Food Science &amp; Emerging Technologies. 9 (2), 170-175 (2008).</li><li>Kumar, A., Li, S., Cheng, C. M., Lee, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Flow-induced+phase+inversion+of+emulsions+in+tapered+microchannels.">Flow-induced phase inversion of emulsions in tapered microchannels.</a> Lab on a Chip. 16 (21), 4173-4180 (2016).</li><li>Atencia, J., Beebe, D. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Controlled+microfluidic+interfaces.">Controlled microfluidic interfaces.</a> Nature. 437, 648-655 (2005).</li><li>Garstecki, P., Fuerstman, M. J., Stone, H. A., Whitesides, G. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Formation+of+droplets+and+bubbles+in+a+microfluidic+T-junctions+scaling+and+mechanism+of+break-up.">Formation of droplets and bubbles in a microfluidic T-junctions scaling and mechanism of break-up.</a> Lab on a Chip. 6 (3), 437-446 (2006).</li><li>Utada, A. S., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Monodisperse+Double+Emulsions+Generated+from+a+Microcapillary+Device.">Monodisperse Double Emulsions Generated from a Microcapillary Device.</a> Science. 308 (5721), 537-541 (2005).</li><li>Ga&#241;an-Calvo, A. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Generation+of+Steady+Liquid+Microthreads+and+Micron-Sized+Monodisperse+Sprays+in+Gas+Streams.">Generation of Steady Liquid Microthreads and Micron-Sized Monodisperse Sprays in Gas Streams.</a> Physical Review Letters. 80 (2), 285-288 (1998).</li><li>Shah, R. K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Designer+emulsions+using+microfluidics.">Designer emulsions using microfluidics.</a> Materials Today. 11 (4), 18-27 (2008).</li><li>Taylor, G. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Disintegration+of+water+drops+in+an+electric+field.">Disintegration of water drops in an electric field.</a> Proceedings of the Royal Society A, Mathematical, Physical and Engineering Sciences. 280 (1382), (1964).</li><li>Gundabala, V. R., Vilanova, N., Fern&#225;ndez-Nieves, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Current-voltage+characteristic+of+electrospray+processes+in+microfluidics.">Current-voltage characteristic of electrospray processes in microfluidics.</a> Physical Review Letters. 105 (15), 154503 (2010).</li><li>Guerrero, J., Rivero, J., Gundabala, V. R., Perez-Saborid, M., Fern&#225;ndez-Nieves, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Whipping+of+electrified+liquid+jets.">Whipping of electrified liquid jets.</a> Proceedings of the National Academy of Sciences of the United States of America. 111 (38), 13763-13767 (2014).</li><li>Vilanova, N., Gundabala, V. R., Fernandez-Nieves, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Drop+size+control+in+electro-coflow.">Drop size control in electro-coflow.</a> Applied Physics Letters. 99 (2), 021910 (2011).</li><li>Cloupeau, M., Prunet-Foch, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrostatic+spraying+of+liquids:+Main+functioning+modes.">Electrostatic spraying of liquids: Main functioning modes.</a> Journal of Electrostatics. 25 (2), 165-184 (1990).</li><li>Jaworek, A., Krupa, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Main+modes+of+electrohydrodynamic+spraying+of+liquids.">Main modes of electrohydrodynamic spraying of liquids.</a> Third International Conference on Multiphase Flow ICMF. , (1998).</li><li>Juraschek, R., R&#246;llgen, F. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pulsation+phenomena+during+electrospray+ionization.">Pulsation phenomena during electrospray ionization.</a> International Journal of Mass Spectrometry. 177 (1), 1-15 (1998).</li><li>Guerrero, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Emission+modes+in+electro+co-flow.">Emission modes in electro co-flow.</a> Physics of Fluids. 31 (8), 082009 (2019).</li><li>Utada, A. S., Fern&#225;ndez-Nieves, A., Stone, H. A., Weitz, D. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dripping+to+jetting+transitions+in+coflowing+liquid+streams.">Dripping to jetting transitions in coflowing liquid streams.</a> Physical Review Letters. 99 (9), 094502 (2007).</li><li>Castro-Hern&#225;ndez, E., Gundabala, V., Fern&#225;ndez-Nieves, A., Gordillo, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Scaling+the+drop+size+in+coflow+experiments.">Scaling the drop size in coflow experiments.</a> New Journal of Physics. 11, 075021 (2009).</li><li>Godfray, H. C. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Food+Security:+the+challenge+of+feeding+9+billion+people.">Food Security: the challenge of feeding 9 billion people.</a> Science. 327 (5967), 812-818 (2010).</li><li>Labb&#233;, R., Gagnier, D., Kostic, A., Shipp, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+function+of+supplemental+foods+for+improved+crop+establishment+of+generalist+predators+Orius+insidiosus+and+Dicyphus+hesperus.">The function of supplemental foods for improved crop establishment of generalist predators Orius insidiosus and Dicyphus hesperus.</a> Scientific Reports. 8 (1), 17790 (2018).</li><li>Pilkington, L. J., Messelink, G., van Lenteren, J. C., Le Mottee, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=34;Protected+Biological+Control&amp;#34;+-+Biological+pest+management+in+the+greenhouse+industry.">34;Protected Biological Control&amp;#34; - Biological pest management in the greenhouse industry.</a> Biological Control. 52 (3), 216-220 (2010).</li><li>Benson, C. M., Labbe, R. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Exploring+the+Role+of+Supplemental+Foods+for+Improved+Greenhouse+Biological+Control.">Exploring the Role of Supplemental Foods for Improved Greenhouse Biological Control.</a> Annals of the Entomological Society of America. 114 (3), 302-321 (2021).</li><li>Temiz, U., &#214;zt&#252;rk, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Encapsulation+methods+and+use+in+animal+nutrition.">Encapsulation methods and use in animal nutrition.</a> Selcuk Journal of Agricultural and Food Sciences. 32 (3), 624-631 (2018).</li><li>Messelink, G. J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Approaches+to+conserving+natural+enemy+populations+in+greenhouse+crops:+current+methods+and+future+prospects.">Approaches to conserving natural enemy populations in greenhouse crops: current methods and future prospects.</a> BioControl. 59, 377-393 (2014).</li><li>Mu&#241;oz-C&#225;rdenas, K., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Generalist+red+velvet+mite+predator+(Balaustium+sp.)+performs+better+on+a+mixed+diet.">Generalist red velvet mite predator (Balaustium sp.) performs better on a mixed diet.</a> Experimental &amp; Applied Acarology. 62 (1), 19-32 (2014).</li><li>van Lenteren, J. C., Bolckmans, K., K&#246;hl, J., Ravensberg, W. J., Urbaneja, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biological+control+using+invertebrates+and+microorganisms:+plenty+of+new+opportunities.">Biological control using invertebrates and microorganisms: plenty of new opportunities.</a> BioControl. 63, 39-59 (2018).</li><li>Urbaneja-Bernat, P., Alonso, M., Tena, A., Bolckmans, K., Urbaneja, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Sugar+as+nutritional+supplement+for+the+zoophytophagous+predator+Nesidiocoris+tenuis.">Sugar as nutritional supplement for the zoophytophagous predator Nesidiocoris tenuis.</a> BioControl. 58 (1), 57-64 (2013).</li><li>Vila, E., Cabello, T., Guevara-Gonzalez, R., Torres-Pacheco, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Biosystems+Engineering+Applied+to+Greenhouse+Pest+Control.">Biosystems Engineering Applied to Greenhouse Pest Control.</a> Biosystems Engineering: Biofactories for Food Production in the Century XXI. , (2014).</li><li>Riudavets, J., Moerman, E., Vila, E., LodovicaGullino, M., Albajes, R. C., Nicot, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Implementation+of+Integrated+Pest+and+Disease+Management+in+Greenhouses:+From+Research+to+the+Consumer.">Implementation of Integrated Pest and Disease Management in Greenhouses: From Research to the Consumer.</a> Integrated Pest and Disease Management in Greenhouse Crops. Plant Pathology in the 21st Century. , (2020).</li><li>Cohen, A. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Insect diets: Science and technology. Second edition. , (2015).</li><li>Sullivan, M. T., Stone, H. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+role+of+feedback+in+microfluidic+flow-focusing+devices.">The role of feedback in microfluidic flow-focusing devices.</a> Philosophical Transactions of the Royal Society A: Mathematical, Physical, and Engineering Sciences. 366 (1873), 2131-2143 (2008).</li><li>Shang, L., Cheng, Y., Zhao, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Emerging+droplet+microfluidics.">Emerging droplet microfluidics.</a> Chemical Reviews. 117 (12), 7964-8040 (2017).</li><li>Christopher, G. F., Anna, S. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microfluidic+methods+for+generating+continuous+droplet+streams.">Microfluidic methods for generating continuous droplet streams.</a> Journal of Physics D: Applied Physics. 40 (19), 319 (2007).</li><li>Nunes, J. K., Tsai, S. S., Wan, J., Stone, H. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dripping+and+jetting+in+microfluidic+multiphase+flows+applied+to+particle+and+fiber+synthesis.">Dripping and jetting in microfluidic multiphase flows applied to particle and fiber synthesis.</a> Journal of Physics D: Applied Physics. 46 (11), 114002 (2013).</li></ol>

The authors have nothing to disclose.

The protocol to fabricate three different glass-based devices has been described above. In the case of the device to generate simple drops, the flow rate and liquid properties are crucial to generate drops in a controlled manner. Drops will form at the tip in the dripping regime, or at the end of the jet in the jetting regime. The transition from dripping to jetting is parametrized by the dimensionless Weber number, We23. This number represents the ratio between inertial and surface tension forces...

Controlled generation of drops in the micron and nanoscale with a narrow size distribution is a challenging task. These drops are of interest for the engineering of soft materials with many applications in science and technology1,2,3,4,5,6.
The most common devices for the high production rate&#160;of drops are mixers7 and ultrasound emulsificators8. These methods are simple and low cost, but they typically result in polydisperse drops with a wide range of sizes. Hence, additional steps are required to produce monodisperse samples. Microfluidic devices can be designed differently to provide an efficient way to drop formation. Additionally, the usually low flow rates involved (i.e., low Reynolds number) allow for great&#160;control over the fluid flow.
While microfluidic devices are commonly made using lithographic techniques with poly(dimethyl) siloxane (PDMS), this manuscript focuses on glass-based capillary devices. PDMS devices are usually chosen for their ability to design complex channel patterns and because of their scalability. Glass devices, on the contrary, are rigid and have greater solvent resistance than their PDMS counterparts. Additionally, glass can be modified to change its wettability, which allows controlling the generation of complex emulsions. Being able to independently treat the nozzle and channel walls enables the formation of drops in a controlled and reproducible manner, while assuring&#160;the stability of the resultant emulsions if the drops were to touch the walls9; otherwise the drops may coalesce and accumulate at the wall. Another difference between these two types of devices is that in glass-based devices, the flow is three-dimensional, while it is planar in conventional PDMS devices. This fact minimizes the drop contact with the channel walls so that the influence of contact lines can be neglected10, thereby protecting the stability of multiple emulsion drops.
<img alt="Figure 1" class="xfigimg" src="/files/ftp_upload/63376/63376fig01.jpg" /> 
Figure 1: Different microfluidic device configurations. Sketches of (A) a T-junction, (B) a coflowing device, and (C) a flow-focusing device. <a href="https://www.jove.com/files/ftp_upload/63376/63376fig01large.jpg" target="_blank">Please click here to view a larger version of this figure.</a>
There are three main geometries used, namely T-junction11, flow focusing12,13, and coflow14. In the T-junction geometry, the dispersed phase contained in the channel perpendicularly intersects the main channel which houses the continuous phase. The shear stress exerted by the continuous phase breaks the incoming dispersed liquid resulting in drops. The generated drops are limited in lower size by the dimensions of the main channel11. In the flow-focusing geometry, the two fluids are forced through a small orifice that is located in front of the injection tube. The result is&#160;the formation of a jet, which is much smaller than the injection tube12,13. Finally, the coflow geometry has a configuration characterized by the coaxial flow of two immiscible fluids14. In general, dripping and jetting can be observed depending on the operating conditions. The dripping regime occurs at low flow rates and the resulting droplets are very monodisperse and have a diameter proportional to the tip size. The drawback is its low production frequency. The jetting regime occurs at higher flow rates as compared to the dripping regime. In this case, the drop diameter is directly proportional to the diameter of the jet which can be much smaller than the diameter of the tip under the right conditions.
An alternative to these hydrodynamic approaches relies on the additional use of electric forces. Electrospray is a well-known and widely used technique for generating droplets. It is based on the principle that a liquid with a finite electrical conductivity will deform in the presence of a strong electric field. The liquid will eventually adopt a conical shape resulting from&#160;the balance between electric and surface tension stresses15. The process starts with the electric field inducing an electric current in the liquid that causes charges to accumulate at the surface. The presence of the electric field results in an electric force on these charges, which drags the liquid along, elongating the meniscus in the direction of the field. Under different conditions, the meniscus can either shed the charged drops or may emit one or several jets which then break into drops15. Although these electrically assisted microfluidic methods naturally allow the generation of small drops, they suffer from a lack of a steady-state operation that compromises the emulsion monodispersity. The resultant charged drops tend to discharge on the confining walls and/or anywhere in the device where the electric potential is lower than the imposed external voltage. Thus, the electrified meniscus becomes unstable, ultimately emitting drops in a chaotic way and causing their uncontrolled production and loss of monodispersity.
In electro-coflow, the electrical and hydrodynamic stresses are coupled in a coflow microfluidic device16 similar to the one used for generating double emulsions12. Two main features allow electro-coflow to be successful in reaching a steady-state emission regime: (i) the dispersed phase is ejected into another coflowing viscous liquid, and (ii) the use of a liquid counter-electrode or ground. Having a flowing outer liquid has proven to change the geometric properties of the drop emission process17. The liquid counter-electrode allows the discharge and extraction of the resultant drops, assuring the steady-state generation of drops. In addition, by exploiting the balance of electrical and hydrodynamic forces, the resultant drop sizes can potentially vary within a&#160;wider range than the sizes that can be covered by any of the previously mentioned techniques.
This detailed video protocol is intended to help new practitioners in the&#160;use and fabrication of glass-based microfluidics.

<table border="1" fo:keep-together.within-page="1" fo:keep-with-next.within-page="always">
	<tbody>
		<tr>
			<td>2-[methoxy(polyethyleneoxy)6-9propyl] trimethoxysilane.</td>
			<td>Gelest</td>
			<td>SIM6492.7</td>
			<td></td>
		</tr>
		<tr>
			<td>Ceramic tile</td>
			<td>Sutter</td>
			<td>CTS</td>
			<td></td>
		</tr>
		<tr>
			<td>Ethylene glycol</td>
			<td>Fisher</td>
			<td>BP230</td>
			<td>These can be found at other companies like Sigma-Aldrich</td>
		</tr>
		<tr>
			<td>Hexane</td>
			<td>Sigma- Aldrich</td>
			<td>34859</td>
			<td>Available in other vendors</td>
		</tr>
		<tr>
			<td>ITW Polymers Adhesives Devcon 5 Minute Epoxy Adhesive 25 mL Dev-Tube</td>
			<td>Ellsworth adhesives</td>
			<td>470740</td>
			<td></td>
		</tr>
		<tr>
			<td>Microforge</td>
			<td>Narishige</td>
			<td>MF 830</td>
			<td></td>
		</tr>
		<tr>
			<td>Micropipette puller</td>
			<td>Sutter</td>
			<td>P97</td>
			<td></td>
		</tr>
		<tr>
			<td>Microscope slides</td>
			<td>Fisher</td>
			<td>12-544-1</td>
			<td>Available in other vendors</td>
		</tr>
		<tr>
			<td>Needle 20 Gauge, .0255&#34; ID, .0355&#34; OD, 1/2&#34; Long</td>
			<td>McMaster</td>
			<td>75165A677</td>
			<td></td>
		</tr>
		<tr>
			<td>SDS</td>
			<td>Sigma-aldrich</td>
			<td>428015</td>
			<td>Surfactant</td>
		</tr>
		<tr>
			<td>Silicone oil</td>
			<td>Clearco</td>
			<td>PSF-10cSt</td>
			<td>The catalog number correspond to the 10cSt viscosity oil. Different viscosity oils can be found at this company</td>
		</tr>
		<tr>
			<td>Span 80</td>
			<td>Fisher</td>
			<td>S0060500G</td>
			<td>non-ionic surfactant</td>
		</tr>
		<tr>
			<td>Square glass capillary 2mm ID (borosillicate 300 or 600 mm long)</td>
			<td>VitroCom</td>
			<td>S 102</td>
			<td></td>
		</tr>
		<tr>
			<td>Standard Glass Capillaries, 6 in., 2 / 1.12 OD/ID</td>
			<td>World Precision instruments</td>
			<td>1B200-6</td>
			<td>These can be found at other companies like Sutter or Vitrocom</td>
		</tr>
		<tr>
			<td>Syringe pump</td>
			<td>Chemyx</td>
			<td>FUSION 100-X</td>
			<td>This model has a good quality/price ratio</td>
		</tr>
		<tr>
			<td>Syringes (it will depend on the compatibility with the liquids)</td>
			<td>Fisher</td>
			<td></td>
			<td>Catalog number will depend on the size</td>
		</tr>
		<tr>
			<td>Trimethoxy(octyl)silane</td>
			<td>Sigma- Aldrich</td>
			<td>376221</td>
			<td>Available in other vendors</td>
		</tr>
		<tr>
			<td>Tubing ( it will depend on the compatibility with the liquids)</td>
			<td>Scientific commodities</td>
			<td>BB3165-PE/5</td>
			<td>This reference is for polyethylene micro tubing. The size fits the needle size listed here</td>
		</tr>
	</tbody>
</table>

glass-based devices to generate drops and emulsions

In this manuscript, three different step-by-step protocols to generate highly monodisperse emulsion drops using glass-based microfluidics are described. The first device is built for the generation of simple drops driven by gravity. The second device is designed to generate emulsion drops in a coflowing scheme. The third device is an extension of the coflowing device with the addition of a third liquid that acts as an electric ground, allowing the formation of electrified drops that subsequently discharge. In this setup, two of the three liquids have an appreciable electrical conductivity. The third liquid mediates between these two and is a dielectric. A&#160;voltage difference applied between the two conducting liquids creates an electric field that couples with hydrodynamic stresses of the coflowing liquids, affecting the jet and drop formation process. The addition of the electric field provides a path to generate smaller drops than in simple coflow devices and for generating particles and fibers with a wide range of sizes.

In this manuscript, three different devices have been designed to generate drops. We have generated drops with a size of (3.29 &#177; 0.08) mm&#160;(Figure 4B) and (1.75 &#177; 0.04) mm&#160;(Figure 4C) using the device described in step 1. The emulsion drops can be generated using the coflow and the electro-coflow devices. For the latter, we show dripping in Figure 9, while cone-jet and whipping modes are shown in <strong class="xf...

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Glass-Based Devices to Generate Drops and Emulsions

Here, a protocol to manufacture glass-based microfluidic devices used for generating highly monodisperse emulsions with controlled drop size is presented.

In this manuscript, three different step-by-step protocols to generate highly monodisperse emulsion drops using glass-based microfluidics are described. ...

This protocol describes how to fabricate glass based microfluidic devices in detail. Following this protocol will allow anyone without experience to build and use these devices. With glass, we can easily render surfaces hydrophobic or hydrophilic thus making glass based devices attractive for generating stable emulsion drops.There are multiple applications for drops and emulsions in fields as diverse as cosmetic, the food industry, and drug delivery. In this manuscript, we emphasize a bit more on their use in intensive agriculture. We recommend being patient at each step.Also check that the tip is clean and not broken. Do not wait until the device is completed to check these. For making simple drops, use a glass base made with a microscope slide to build the device.For the tip, use one millimeter diameter round glass capillaries. Next, use a 20 gauge syringe needle to facilitate introducing the liquid into the capillary. Using a razor blade or a scalpel, carve a hole equal to the size of the outer diameter of the capillary at the base of the needle.Rinse the needle with water to remove any dust and fibers from cutting and allow the needle to air dry. To assemble the device, glue the round capillary to the microscope slide by placing the tip of the capillary one to two centimeters outside the end of the microscope slide and adding a dab of epoxy at the center of the capillary such that it will not interfere with the field of vision or with the syringe needle. Next, place the syringe needle such that the end of the capillary sits at the center of the needle and put a small amount of hardened epoxy around the rim at the bottom of the needle.After a few minutes, apply the second layer of fresh epoxy, covering the base of the needle and avoiding the hole. Then, cover the hole with hardened epoxy to prevent the epoxy from flowing inside the needles. For generating drops, place the device in a vertical position using a clamp such that the tip is facing down like a kitchen faucet.Use a syringe pump or a pressure driven setup to pump the liquid into the device. For making emulsion drops, use a five centimeter long square capillary for the outer liquid and a one millimeter diameter round capillary. Ensure that the length of the round capillary is several centimeters longer than the square capillary.Match the outer diameter of the round capillary to the inner size of the square capillary to ensure that both capillaries are coaxially aligned. Next, to assemble the device, glue the square capillary to the microscope slide by placing the tip of the capillary one to two centimeters outside the end of the microscope slide and applying a dab of epoxy at the center of the capillary. Wait until the epoxy cures completely.Then, introduce the round capillary into the square capillary such that the end of the round capillary on the slide is a few centimeters from the end of the square capillary. The other end of the round capillary which is inside the square capillary should be at a distance approximately equal to the diameter of the round capillary from the end of the square capillary. Glue the round capillary using a dab of epoxy at mid-distance between the end of the round capillary and the beginning of the square capillary and wait until the epoxy cures completely.To house the capillary in the needle base, carve a hole at the base of the round cap which is the size of the outer diameter of the capillary. Then, to fit the other needle at the end of the square capillary, carve a round and a square hole at the base of the needle to accommodate the joint. After cutting, rinse the needles with water and let them air dry.Then glue them as demonstrated earlier. To build the microfluidic device, cut two small pieces of a slide and using epoxy, glue them to two microscope slides to keep the slides together. Wait until the epoxy cures completely.Using a diamond scribe, cut the square capillary to a length of approximately four centimeters. Use the square capillary with an inner side that matches the outer diameter of the round capillary to align the capillaries coaxially. For this protocol, use a two millimeter side capillary.Then, using a pipette pulling machine, pull a round capillary to obtain two half capillaries with a thin tip. Next, using a micro forge, cut the tip of one of the half capillaries to the desired diameter. To use the other half as a collector capillary, cut the pulled tip so that the original flat ends are recovered.Glue the square capillary to the slides. Do not put the capillary in the middle of the slides as the joints of the slides should not be in the viewing region. Then, place the tip and collector capillary inside the square capillary around two millimeters apart.To avoid the joint, place the ends of both the tip and collector capillaries on the same side. To fabricate connections to the open ends of the capillaries, place four needles covering these ends and glue the needles as demonstrated earlier. To test the device for leaks, close two of the needles using a bent piece of tubing held by a binder clip.Then, using a syringe, manually pump deionized water into the device through one of the needles using the last needle as an exit. If no leakages are observed, pump the water through the next needle repeating the process for all four needles. If a leak is found, thoroughly dry the device, apply epoxy, and wait for one hour before testing again.After confirming no leakage, fill the device with experimental liquid and remove air bubbles. Then connect the inner liquid or dispersed phase syringe to needle one, the outer liquid or continuous phase to needle two, and the collector liquid or counter electrode to needle four. Needle three is for the exit.Finally, connect the power supply to the needles feeding the inner and collector liquids to set a potential difference between the tip and collector liquid. In this study, approximately 3.3 millimeter silicone oil drops were generated using a 580 micrometer tip. And 1.75 millimeter drops were generated using an 86 micrometer tip.Additionally, emulsion drops were generated using electro co flow devices in the dripping, cone jet, and whipping modes. In these results, the same liquid is used as inner and collector liquids. However, if the experiments goal is collecting these drops, a different conducting liquid should be used as a collector.Be patient and wait until the epoxy cures. If it gets wet, it will never dry and you will need to start the entire procedure again. Multiple emulsions can be generated using in combinations of flow focus in a scheming series.Each extra scheme will add a shell to the motion drop. Flow focusing sections can also be used.

glass-based-devices-to-generate-drops-and-emulsions

Research

JoVE Journal

Engineering

2.5K vues.  Augusta University. Ce protocole décrit en détail comment fabriquer des dispositifs microfluidiques à base de verre. Suivre ce protocole permettra à toute personne sans expérience de construire et d’utiliser ces appareils. Avec le verre, nous pouvons facilement rendre les surfaces hydrophobes ou hydrophiles, rendant ainsi les appareils à base de verre attrayants pour générer des gouttes d’émulsion stables.Il existe de multiples applications pour les gouttes et les émulsions dans des domaines...