Incubation of Soil in Liquid Broth and Transferring the Supernatant to the Solid Medium
2:34
Detection of Yeasts, Streaking for Single Colonies, and Identification of Yeast Species
4:04
Isolation of Aspergillus fumigatus from Soil
7:32
Results: Isolation and Characterization of Soil Yeast and Aspergillus fumigatus Populations
9:37
Conclusion
Transcription
Our protocol investigates yeast and mold diversity in soil using soil samples obtained from different climatic regions. This provides insights into environmental yeast diversity and helps track pathogenic species. The protocol offers a cost-effect
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This protocol is an effective, speedy method of culturing yeasts and the mold Aspergillus fumigatus from large sets of soil samples in as little as 7 days. The methods can be easily modified to accommodate a range of incubation media and temperatures as needed for experiments.