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Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes

DOI :

10.3791/65472-v

July 14th, 2023

July 14th, 2023

927 Views

1Unità di Genetica, Dipartimento di Biologia, University of Pisa, 2Department of Women-Child-Newborn Obstetrics and Gynaecology, Foundation IRCCS Ca' Granda Ospedale Maggiore Policlinico

This protocol presents a method to assess the formation and repair of DNA double-strand breaks through the simultaneous detection of γH2AX and 53BP1 foci in interphase nuclei of bleomycin-treated human peripheral lymphocytes.

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Dual Immunofluorescence

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