אנו מודים לד&quot;ר Krishanu ריי למניות תסיסנית, Tarjani Agarwal לשמירת תסיסנית כלוב, פיטר Juo לnuIs25 וCGC לג זני elegans. SPK עשה jsIs609 במעבדתו של מייקל התשיעייה. אנו מודים Arpan Agnihotri (Pilani BITS) על עזרתו בזמן לשגות הדמיה של תחבורת מיטוכונדריה של jsIs609 בעלי חיים בהתקני microfluidic. אנו מודים לד&quot;ר Vatsala Thirumalai וסורייה פראקאש שספק לנו עוברי דג זברה. אנו מודים לד&quot;ר קרישנה וCIFF בבנק המרכזי לאומי לשימוש במיקרוסקופ confocal הדיסק מסתובב נתמך על ידי משרד מדע וטכנולוגיה, המרכז לננוטכנולוגיה (מס SR/55/NM-36-2005). אנו מודים גם לKaustubh ראה, ו &#39;Venkatraman וChetana Sachidanand לדיונים. עבודה זו מומנה על ידי מלגת DBT פוסט הדוקטורט (SM), ערכת מסלול מהיר DST (SM) וDBT מענק ל( SPK). SA נתמך על ידי מענקי DST וCSIR לSPK.

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אין ניגודי האינטרסים הכריזו.

התקני microfluidic PDMS הם שקופים אופטי ולכן ניתן להשתמש בו לרזולוציה גבוהה בתחום הדמית vivo של כל אורגניזם מודל שקוף / שקוף. התכנון שלנו הוא מתאים להדמית הגדלה גבוהה זמנית spatio של אירועים הסלולר ומשנה הסלולר בבעלי חיים שלמים. Microfabrication באמצעות טכניקות ליתוגרפיה רכות מ...

<table border="1" style=";text-align:right;direction:rtl"><tbody><tr><td> שם המגיב </td><td> חברה </td><td> מספר קטלוגים </td><td> תגובות (אופציונלי) </td></tr><tr><td> פרוסות סיליקון </td><td> רקיק אוניברסיטה </td><td> 150 מ&quot;מ (100) Mech כיתת SSP סי </td><td></td></tr><tr><td> Clewin תוכנה </td><td> WieWeb תוכנה </td><td> גרסה 2.90 </td><td></td></tr><tr><td> פלוטר ליזר </td><td> הדמית קו דקה </td><td> 65024 DPI </td><td></td></tr><tr><td> HMDS </td><td> סיגמה אולדריץ </td><td> 440191-100 מ&quot;ל </td><td></td></tr><tr><td> SU8 </td><td> Microchem </td><td> SU8-2025, SU8-2050 </td><td></td></tr><tr><td> מפתחים </td><td> Microchem </td><td> SU8 מפתחים </td><td></td></tr><tr><td> Silane </td><td> סיגמה אולדריץ </td><td> 448931-10G </td><td></td></tr><tr><td> PDMS </td><td> Dow Corning </td><td> Sylgard 184 </td><td></td></tr><tr><td> מנורת UV </td><td> אוריאל </td><td> 66943 </td><td> 200W הכספי אוריאל אור </td></tr><tr><td> תנור אוויר חם </td><td> מכשירי Ultra </td><td> Custom made </td><td> הגדר ב 50 מעלות צלזיוס </td></tr><tr><td> פלטה </td><td> ציוד מעבדת איקא </td><td> 3810000 </td><td> <a href="http://www.ika.com" target="_blank">http://www.ika.com</a> </td></tr><tr><td> שואב פלזמה </td><td> Harrick פלזמה </td><td> PDC-32G </td><td></td></tr><tr><td> טווה </td><td> מערכות ייצור מוליכים למחצה </td><td> SPIN150-NPP </td><td> <a href="http://www.SPS-Europe.com" target="_blank">www.SPS-Europe.com</a> </td></tr><tr><td> תלוש כיסוי זכוכית </td><td> זהב חותם </td><td> 22 22 מ&quot;מ X, עובי מס &#39;1 </td><td></td></tr><tr><td>סי אלגנס </td><td> Caenorhabditis גנטיקת המרכז (CGC) </td><td> e1265, ayIs4 </td><td></td></tr><tr><td> דרוזופילה </td><td> בלומינגטון </td><td> P {chaGAL4} / ארגון נוער קתולי, כטב&quot;מ-syt.eGFP </td><td></td></tr><tr><td> דג זברה </td><td> סוג פראי הודי </td><td> סוג פרוע </td><td></td></tr><tr><td> צינור Tygon </td><td> סיגמא </td><td> Z279803 </td><td></td></tr><tr><td> מחט מייקרו </td><td> סיגמא </td><td> Z118044 </td><td> חותך לחתיכות 1 סנטימטר </td></tr><tr><td> 3-way ברזלים </td><td> סיגמא </td><td> S7521 </td><td></td></tr><tr><td> אגרופן אריס </td><td> סיגמא </td><td> Z708631 </td><td></td></tr><tr><td> גז חנקן דחוס </td><td> ספק גז מקומי </td><td></td><td> השתמש בוסת, כדי לשלוט בלחץ </td></tr><tr><td> מיקרוסקופ סטריאו &lt;/ Td&gt; <td> ניקון </td><td> SMZ645 </td><td></td></tr><tr><td> מיקרוסקופ confocal </td><td> תידור ואולימפוס </td><td> Yokogawa ספינינג דיסק confocal מיקרוסקופ </td><td></td></tr><tr><td> ImageJ </td><td> המכונים הלאומיים לבריאות </td><td> <a href="http://www.rsbweb.nih.gov/ij" target="_blank">www.rsbweb.nih.gov / ij</a> </td><td> Java תכנית עיבוד תמונה מבוססת </td></tr></tbody></table>

simple microfluidic devices for in vivo imaging of c. elegans, drosophila and zebrafish

מכשירי fluidic מייקר מפוברק לספק סביבת מייקרו נגישה למחקרי vivo על אורגניזמים קטנים. תהליכי ייצור פשוטים זמינים עבור התקני microfluidic באמצעות טכניקות ליתוגרפיה רכות 1-3. התקני microfluidic שמשו במשך הדמית משנה הסלולר 4,5, in vivo ליזר microsurgery 2,6 וסלולרית הדמיה 4,7. בחי ההדמיה דורשת קיבוע של אורגניזמים. זו הושגה באמצעות שאיבה 5,8, ערוצים מחודדים 6,7,9, ממברנות deformable 2-4,10, יניקה עם קירור נוסף 5, הרדמת גז 11, ג&#39;לים רגיש לטמפרטורה 12, דבק cyanoacrylate 13 והרדמה כגון levamisole 14 , בן 15. הרדמה נפוצה להשפיע על הולכה סינפטית 16,17 וידועים יש השפעות מזיקות על 4 הובלה עצביות משנה הסלולר. ברחוב זהג&#39;ודי אנחנו מדגימים קרום בסיס פולי דימתיל-siloxane מכשיר (PDMS) המאפשר קיבוע ללא הרדמה של אורגניזמים מודל גנטיים תקינים כגון elegans Caenorhabditis (סי אלגנס), זחלי דרוזופילה וזחלי דג זברה. אורגניזמים מודל אלו מתאימים למחקרי vivo במכשירי microfluidic בגלל קוטרם הקטן והגופים שקופים אופטי או שקופים. קטרי גוף לנוע בין ~ 10 מיקרומטר ל ~ 800 מיקרומטר לשלבים מוקדמים של זחל ג זחלי elegans ודג זברה ודורש התקני microfluidic בגדלים שונים כדי להשיג קיבוע מלא ל הדמיה ברזולוציה גבוהה זמן לשגות. אורגניזמים אלה משותקים באמצעות לחץ המופעל על ידי גז חנקן דחוס דרך עמודת נוזל וצלם באמצעות מיקרוסקופ הפוך. בעלי חיים ששוחררו מהמלכודת לחזור לתנועה רגילה בתוך 10 דקות. אנחנו מדגימים ארבעה יישומים של הדמית זמן לשגות בג elegansכלומר, תחבורת הדמית המיטוכונדריה בתאי עצב, תחבורת שלפוחית ​​מראש הסינפטי בתחבורת תחבורה-פגומת מוטציה, קולטן הגלוטמט וחלוקת תא neuroblast ש. נתונים שהתקבלו מסרטים כאלה מראים שקיבוע microfluidic הוא אמצעי שימושי ומדויק של רכישה בנתוני vivo של אירועים הסלולר ומשנה הסלולר בהשוואה לחיות מורדמות (האיור 1J ו3C-F 4). מידות מכשיר שונו כדי לאפשר הדמית זמן לשגות בשלבים שונים של ג elegans, תחילה זחלי instar דרוזופילה וזחלי דג זברה. הובלה של שלפוחית ​​מסומנים synaptotagmin מתויג עם GFP (syt.eGFP) בנוירונים חושיים תערוכות תנועה מכוונת של סמני שלפוחית ​​סינפטיים לידי ביטוי בתאי עצב תחושתיים כולינרגיות בזחלי תסיסנית שלמים 1 instar. מכשיר דומה כבר נעשה שימוש כדי לבצע הדמית זמן לשגות של פעימות לב ב~ הפרית הודעת שעה 30 (hpf)דג זברת זחלים. נתונים אלו מראים כי מכשירים הפשוטים שפתחנו ניתן ליישם במגוון רחב של מערכות מודל ללמוד כמה תאי תופעות ביולוגיות וההתפתחותי בגוף חי.

מכשיר הקיבוע הוא בלוק bilayer PDMS המפוברק על ידי שתי שכבות מליטות: שכבת זרימה (שכבה 1) ושכבת שליטה (שכבה 2) כפי שמוצג באיור 1. המלכודת הראשית מחוברת לבלון גז חנקן באמצעות רגולטור וזין תחנה 3-דרך ליישם את הלחץ דרוש (3-14 psi) על גבי הקרום דרך עמודת נוזל (איור 1 א). הק...

Watch this Scientific Journal Video about Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish at JoVE.com

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

מכשיר microfluidic פשוט פותח כדי לבצע הרדמה חופשיה<em&gt; In vivo</em&gt; הדמיה של<em&gt; ג elegans</em&gt;, שלם<em&gt; דרוזופילה</em&gt; זחלים וזחלי דג זברה. המכשיר מנצל PDMS קרום deformable לשתק אורגניזמים המודל הבא על מנת לבצע זמן לשגות הדמיה של תהליכים רבים כגון קצב לב, חלוקת תא והובלה עצבית משנה הסלולר. אנו מדגימים את השימוש במכשיר זה ולהראות דוגמאות לסוגים שונים של נתונים שנאספו ממערכות מודל שונות.

התקני microfluidic פשוטים עבור<em&gt; In vivo</em&gt; הדמיה של<em&gt; ג elegans</em&gt;,<em&gt; דרוזופילה</em&gt; ודג זברה

Micro fabricated fluidic devices provide an accessible micro-environment for in vivo studies on small organisms. Simple fabrication processes are ...

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Research

JoVE Journal

Bioengineering

Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

17.0K Views.  NCBS-TIFR. מכשיר microfluidic פשוט פותח כדי לבצע הרדמה חופשיה In vivo הדמיה של ג elegans, שלם דרוזופילה זחלים וזחלי דג זברה. המכשיר מנצל PDMS קרום deformable לשתק אורגניזמים המודל הבא על מנת לבצע זמן לשגות הדמיה של תהליכים רבים כגון קצב לב, חלוקת תא והובלה עצבית משנה הס?...

Video: Simple Microfluidic Devices for in vivo Imaging of C. elegans, Drosophila and Zebrafish

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Microfluidic devices have advanced cell studies by providing a dynamic fluidic environment on the scale of the cell for studying, manipulating, sorting and counting cells. However, manipulating the cell within the fluidic domain remains a challenge and requires complicated fabrication protocols for forming valves and electrodes, or demands specialty equipment like optical tweezers. Here, we demonstrate that conventional printed circuit boards (PCB) can be used for the non-contact manipulation of cells by employing dielectrophoresis (DEP) for bead and cell manipulation in laminar flow fields for bioactuation, and for cell and bead separation in multichannel microfluidic devices. First, we present the protocol for assembling the DEP electrodes and microfluidic devices, and preparing the cells for DEP. Then, we characterize the DEP operation with polystyrene beads. Lastly, we show representative results of bead and cell separation in a multichannel microfluidic device. In summary, DEP is an effective method for manipulating particles (beads or cells) within microfluidic devices.

Dielectrophoresis (DEP) is an effective method to manipulate cells. Printed circuit boards (PCB) can provide inexpensive, reusable and effective electrodes for contact-free cell manipulation within microfluidic devices. By combining PDMS-based microfluidic channels with coverslips on PCBs, we demonstrate bead and cell manipulation and separation within multichannel microfluidic devices.

חרוזים ועל הפרדת תאים ריבוי ערוצים מכשירים microfluidic שימוש dielectrophoresis ו זרימה למינרית

Microfluidic devices have advanced cell studies by providing a dynamic fluidic environment on the scale of the cell for studying, manipulating, sorting ...

A Microfluidic Technique to Probe Cell Deformability

Here we detail the design, fabrication, and use of a microfluidic device to evaluate the deformability of a large number of individual cells in an efficient manner. Typically, data for ~102 cells can be acquired within a 1 hr experiment. An automated image analysis program enables efficient post-experiment analysis of image data, enabling processing to be complete within a few hours. Our device geometry is unique in that cells must deform through a series of micron-scale constrictions, thereby enabling the initial deformation and time-dependent relaxation of individual cells to be assayed. The applicability of this method to human promyelocytic leukemia (HL-60) cells is demonstrated. Driving cells to deform through micron-scale constrictions using pressure-driven flow, we observe that human promyelocytic (HL-60) cells momentarily occlude the first constriction for a median time of 9.3 msec before passaging more quickly through the subsequent constrictions with a median transit time of 4.0 msec per constriction. By contrast, all-trans retinoic acid-treated (neutrophil-type) HL-60 cells occlude the first constriction for only 4.3 msec before passaging through the subsequent constrictions with a median transit time of 3.3 msec. This method can provide insight into the viscoelastic nature of cells, and ultimately reveal the molecular origins of this behavior.

We demonstrate a microfluidics-based assay to measure the timescale for cells to transit through a sequence of micron-scale constrictions.

טכניקת Microfluidic לחקור deformability הסלולרי

Here we detail the design, fabrication, and use of a microfluidic device to evaluate the deformability of a large number of individual cells in an ...

Layer-by-layer Collagen Deposition in Microfluidic Devices for Microtissue Stabilization

Although microfluidics provides exquisite control of the cellular microenvironment, culturing cells within microfluidic devices can be challenging. 3D culture of cells in collagen type I gels helps to stabilize cell morphology and function, which is necessary for creating microfluidic tissue models in microdevices. Translating traditional 3D culture techniques for tissue culture plates to microfluidic devices is often difficult because of the limited channel dimensions. In this method, we describe a technique for modifying native type I collagen to generate polycationic and polyanionic collagen solutions that can be used with layer-by-layer deposition to create ultrathin collagen assemblies on top of cells cultured in microfluidic devices. These thin collagen layers stabilize cell morphology and function, as shown using primary hepatocytes as an example cell, allowing for the long term culture of microtissues in microfluidic devices.

The creation of functional microtissues within microfluidic devices requires the stabilization of cell phenotypes by adapting traditional cell culture techniques to the limited spatial dimensions in microdevices. Modification of collagen allows the layer-by-layer deposition of ultrathin collagen assemblies that can stabilize primary cells, such as hepatocytes, as microfluidic tissue models.

קולגן שכבת הפקדת-ידי שכבה במכשירי microfluidic לMicrotissue ייצוב

Although microfluidics provides exquisite control of the cellular microenvironment, culturing cells within microfluidic devices can be challenging. 3D ...

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

The behavior of directional cell migration under a direct current electric-field (dcEF) is referred to as electrotaxis. The significant role of physiological dcEF in guiding cell movement during embryo development, cell differentiation, and wound healing has been demonstrated in many studies. By applying microfluidic chips to an electrotaxis assay, the investigation process is shortened and experimental errors are minimized. In recent years, microfluidic devices made of polymeric substances (e.g., polymethylmethacrylate, PMMA, or acrylic) or polydimethylsiloxane (PDMS) have been widely used in studying the responses of cells to electrical stimulation. However, unlike the numerous steps required to fabricate a PDMS device, the simple and rapid construction of the acrylic micro&#64258;uidic chip makes it suitable for both device prototyping and production. Yet none of the reported devices facilitate the efficient study of the simultaneous chemical and dcEF effects on cells. In this report, we describe our design and fabrication of an acrylic-based multichannel dual-electric-field (MDF) chip to investigate the concurrent effect of chemical and electrical stimulation on lung cancer cells. The MDF chip provides eight combinations of electrical/chemical stimulations in a single test. The chip not only greatly shortens the required experimental time but also increases accuracy in electrotaxis studies.

Many microfluidic devices have been developed for use in the study of electrotaxis. Yet, none of these chips allows the efficient study of the simultaneous chemical and electric-field (EF) effects on cells. We developed a polymethylmethacrylate-based device that offers better-controlled coexisting EF and chemical stimulation for use in electrotaxis research.

Electrotaxis מחקרים של תאי סרטן ריאות באמצעות רב-ערוצי דו-חשמלי-שדה שבב microfluidic

The behavior of directional cell migration under a direct current electric-field (dcEF) is referred to as electrotaxis. The significant role of ...

Using Adhesive Patterning to Construct 3D Paper Microfluidic Devices

We demonstrate the use of patterned aerosol adhesives to construct both planar and nonplanar 3D paper microfluidic devices. By spraying an aerosol adhesive through a metal stencil, the overall amount of adhesive used in assembling paper microfluidic devices can be significantly reduced. We show on a simple 4-layer planar paper microfluidic device that the optimal adhesive application technique and device construction style depends heavily on desired performance characteristics. By moderately increasing the overall area of a device, it is possible to dramatically decrease the wicking time and increase device success rates while also reducing the amount of adhesive required to keep the device together. Such adhesive application also causes the adhesive to form semi-permanent bonds instead of permanent bonds between paper layers, enabling single-use devices to be non-destructively disassembled after use. Nonplanar 3D origami devices also benefit from the semi-permanent bonds during folding, as it reduces the likelihood that unrelated faces may accidently stick together. Like planar devices, nonplanar structures see reduced wicking times with patterned adhesive application vs uniformly applied adhesive.

We demonstrate the use of patterned aerosol adhesives to construct 3D paper microfluidic devices. This method of adhesive application forms semi-permanent bonds between layers, enabling single-use devices to be non-destructively disassembled after use and to ease folding complex nonplanar structures.

באמצעות דפוסים דבקים כדי לבנות התקני 3D נייר Microfluidic

We demonstrate the use of patterned aerosol adhesives to construct both planar and nonplanar 3D paper microfluidic devices. By spraying an aerosol ...

Multi-step Variable Height Photolithography for Valved Multilayer Microfluidic Devices

Microfluidic systems have enabled powerful new approaches to high-throughput biochemical and biological analysis. However, there remains a barrier to entry for non-specialists who would benefit greatly from the ability to develop their own microfluidic devices to address research questions. Particularly lacking has been the open dissemination of protocols related to photolithography, a key step in the development of a replica mold for the manufacture of polydimethylsiloxane (PDMS) devices. While the fabrication of single height silicon masters has been explored extensively in literature, fabrication steps for more complicated photolithography features necessary for many interesting device functionalities (such as feature rounding to make valve structures, multi-height single-mold patterning, or high aspect ratio definition) are often not explicitly outlined. 
Here, we provide a complete protocol for making multilayer microfluidic devices with valves and complex multi-height geometries, tunable for any application. These fabrication procedures are presented in the context of a microfluidic hydrogel bead synthesizer and demonstrate the production of droplets containing polyethylene glycol (PEG diacrylate) and a photoinitiator that can be polymerized into solid beads. This protocol and accompanying discussion provide a foundation of design principles and fabrication methods that enables development of a wide variety of microfluidic devices. The details included here should allow non-specialists to design and fabricate novel devices, thereby bringing a host of recently developed technologies to their most exciting applications in biological laboratories.

Multilayer microfluidic devices often involve the fabrication of master molds with complex geometries for functionality. This article presents a complete protocol for multi-step photolithography with valves and variable height features tunable to any application. As a demonstration, we fabricate a microfluidic droplet generator capable of producing hydrogel beads.

פוטו-ליתוגרפיה גובה רב שלבי משתנה עבור Valved רב שכבתי מכשירים microfluidic

Microfluidic systems have enabled powerful new approaches to high-throughput biochemical and biological analysis. However, there remains a barrier to ...

Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays

Paper wicks fluids autonomously due to capillary action. By patterning paper with hydrophobic barriers, the transport of fluids can be controlled and directed within a layer of paper. Moreover, stacking multiple layers of patterned paper creates sophisticated three-dimensional microfluidic networks that can support the development of analytical and bioanalytical assays. Paper-based microfluidic devices are inexpensive, portable, easy to use, and require no external equipment to operate. As a result, they hold great promise as a platform for point-of-care diagnostics. In order to properly evaluate the utility and analytical performance of paper-based devices, suitable methods must be developed to ensure their manufacture is reproducible and at a scale that is appropriate for laboratory settings. In this manuscript, a method to fabricate a general device architecture that can be used for paper-based immunoassays is described. We use a form of additive manufacturing (multi-layer lamination) to prepare devices that comprise multiple layers of patterned paper and patterned adhesive. In addition to demonstrating the proper use of these three-dimensional paper-based microfluidic devices with an immunoassay for human chorionic gonadotropin (hCG), errors in the manufacturing process that may result in device failures are discussed. We expect this approach to manufacturing paper-based devices will find broad utility in the development of analytical applications designed specifically for limited-resource settings.

We detail a method to fabricate three-dimensional paper-based microfluidic devices for use in the development of immunoassays. Our approach to device assembly is a type of multilayer, additive manufacturing. We demonstrate a sandwich immunoassay to provide representative results for these types of paper-based devices.

ייצור של מכשירי microfluidic נייר מבוססים תלת ממדי עבור Immunoassays

Paper wicks fluids autonomously due to capillary action. By patterning paper with hydrophobic barriers, the transport of fluids can be controlled and ...

Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

This paper reports a microfluidic device made of polydimethylsiloxane (PDMS) with an embedded polycarbonate (PC) thin film to study cell migration under combinations of chemical and oxygen gradients. Both chemical and oxygen gradients can greatly affect cell migration in vivo; however, due to technical limitations, very little research has been performed to investigate their effects in vitro. The device developed in this research takes advantage of a series of serpentine-shaped channels to generate the desired chemical gradients and exploits a spatially confined chemical reaction method for oxygen gradient generation. The directions of the chemical and oxygen gradients are perpendicular to each other to enable straightforward migration result interpretation. In order to efficiently generate the oxygen gradients with minimal chemical consumption, the embedded PC thin film is utilized as a gas diffusion barrier. The developed microfluidic device can be actuated by syringe pumps and placed into a conventional cell incubator during cell migration experiments to allow for setup simplification and optimized cell culture conditions. In cell experiments, we used the device to study migrations of adenocarcinomic human alveolar basal epithelial cells, A549, under combinations of chemokine (stromal cell-derived factor, SDF-1&#945;) and oxygen gradients. The experimental results show that the device can stably generate perpendicular chemokine and oxygen gradients and is compatible with cells. The migration study results indicate that oxygen gradients may play an essential role in guiding cell migration, and cellular behavior under combinations of gradients cannot be predicted from those under single gradients. The device provides a powerful and practical tool for researchers to study interactions between chemical and oxygen gradients in cell culture, which can promote better cell migration studies in more in vivo-like microenvironments.

The control of chemical and oxygen gradients is essential for cell cultures. This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) microfluidic device capable of reliably generating combinations of chemical and oxygen gradients for cell migration studies, which can be practically utilized in biological labs without sophisticated instrumentation.

Polydimethylsiloxane-פוליקרבונט התקני microfluidic הכימי בניצב תחת מחקרי נדידת תאים והדרגות חמצן

This paper reports a microfluidic device made of polydimethylsiloxane (PDMS) with an embedded polycarbonate (PC) thin film to study cell migration under ...

A Simple and Scalable Fabrication Method for Organic Electronic Devices on Textiles

Today, wearable electronics devices combine a large variety of functional, stretchable, and flexible technologies. However, in many cases, these devices cannot be worn under everyday conditions. Therefore, textiles are commonly considered the best substrate to accommodate electronic devices in wearable use. In this paper, we describe how to selectively pattern organic electroactive materials on textiles from a solution in an easy and scalable manner. This versatile deposition technique enables the fabrication of wearable organic electronic devices on clothes.

In this paper, we present a protocol to selectively deposit organic materials on textiles, which allows for the direct integration of organic electronic devices with wearables. The fabricated devices can be fully integrated in textiles, respecting their mechanical appearance and enabling sensing capabilities.

שיטת ייצור פשוט Scalable עבור מכשירים אלקטרוניים אורגניים על טקסטיל

Today, wearable electronics devices combine a large variety of functional, stretchable, and flexible technologies. However, in many cases, these devices ...

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

In the last decade, microfluidic techniques have been applied to study small animals, including the nematode Caenorhabditis elegans, and have proved useful as a convenient live imaging platform providing capabilities for precise control of experimental conditions in real time. In this article, we demonstrate live imaging of individual worms employing WormSpa, a previously-published custom microfluidic device. In the device, multiple worms are individually confined to separate chambers, allowing multiplexed longitudinal surveillance of various biological processes. To illustrate the capability, we performed proof-of-principle experiments in which worms were infected in the device with pathogenic bacteria, and the dynamics of expression of immune response genes and egg laying were monitored continuously in individual animals. The simple design and operation of this device make it suitable for users with no previous experience with microfluidic-based experiments. We propose that this approach will be useful for many researchers interested in longitudinal observations of biological processes under well-defined conditions.

A Microfluidic Platform for Longitudinal Imaging in Caenorhabditis elegans

In this article, we demonstrate live imaging of individual worms employing a custom microfluidic device. In the device, multiple worms are individually confined to separate chambers, allowing multiplexed longitudinal surveillance of various biological processes.

פלטפורמה Microfluidic הדמיה האורך ב Caenorhabditis elegans

In the last decade, microfluidic techniques have been applied to study small animals, including the nematode Caenorhabditis elegans, and have proved ...