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Assay exonuclease הקרינה מבוסס לאפיין DmWRNexo, Orthologue של האדם Progeroid WRN exonuclease, ויישומו nucleases אחר

Exonucleases play critical roles in ensuring genome stability. Loss of WRN exonuclease function results in premature aging. Studying substrates and other requirements of the nuclease in vitro can help elucidate its role in vivo. Here we demonstrate a rapid and reproducible fluorescence-based assay to measure its nuclease activity.

Chapters in this video

0:05

Title

0:54

His-trap Purification

2:24

Nuclease Assay

4:14

Results: The Exonuclease Activity of DmWRNexo

5:51

Conclusion

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