Name: a standardized procedure of dressing management for toxic epidermal necrolysis
Uploaded: 2025-03-14T13:40:00.000+00:00
Duration: 7 min 22 s
Description: Toxic epidermal necrolysis (TEN) is a severe cutaneous adverse drug reaction with high mortality rates, demanding specialized wound care to address ...

<ol>
	<li>Frantz, R., Huang, S., Are, A., Motaparthi, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stevens-Johnson+syndrome+and+toxic+epidermal+necrolysis:+A+review+of+diagnosis+and+management.">Stevens-Johnson syndrome and toxic epidermal necrolysis: A review of diagnosis and management.</a> Medicine (Kaunas). 57 (9), 895 (2021).</li><li>Hu, L., Huang, K., Yu, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Disease+progression+and+treatment+outcome+of+Stevens-Johnson+Syndrome+and+toxic+epidermal+necrolysis.">Disease progression and treatment outcome of Stevens-Johnson Syndrome and toxic epidermal necrolysis.</a> Chinese Journal of Dermatology and Venereology. 36 (03), 290-294 (2022).</li><li>Dodiuk-Gad, R. P., Chung, W. -. H., Valeyrie-Allanore, L., Shear, N. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stevens-Johnson+Syndrome+and+toxic+epidermal+necrolysis:+An+update.">Stevens-Johnson Syndrome and toxic epidermal necrolysis: An update.</a> Am J Clin Dermatol. 16 (6), 475-493 (2015).</li><li>Creamer, D., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=UK+guidelines+for+the+management+of+Stevens-Johnson+syndrome/toxic+epidermal+necrolysis+in+adults+2016.">UK guidelines for the management of Stevens-Johnson syndrome/toxic epidermal necrolysis in adults 2016.</a> J Plast Reconstr Aesthet Surg. 69 (6), 736-741 (2016).</li><li>Jaller, J. A., McLellan, B. N., Balagula, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Wound+management+in+Stevens-Johnson+Syndrome+and+toxic+epidermal+necrolysis.">Wound management in Stevens-Johnson Syndrome and toxic epidermal necrolysis.</a> Curr Dermatol Rep. 9 (1), 58-72 (2020).</li><li>Seminario-Vidal, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Society+of+Dermatology+Hospitalists+supportive+care+guidelines+for+the+management+of+Stevens-Johnson+syndrome/toxic+epidermal+necrolysis+in+adults.">Society of Dermatology Hospitalists supportive care guidelines for the management of Stevens-Johnson syndrome/toxic epidermal necrolysis in adults.</a> J Am Acad Dermatol. 82 (6), 1553-1567 (2020).</li><li>Liang, Y., He, J., Guo, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Functional+hydrogels+as+wound+dressing+to+enhance+wound+healing.">Functional hydrogels as wound dressing to enhance wound healing.</a> ACS Nano. 15 (8), 12687-12722 (2021).</li><li>Liang, Z., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Impact+of+moist+wound+dressing+on+wound+healing+time:+A+meta-analysis.">Impact of moist wound dressing on wound healing time: A meta-analysis.</a> Int Wound J. 20 (10), 4410-4421 (2023).</li><li>Vivcharenko, V., Trzaskowska, M., Przekora, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Wound+dressing+modifications+for+accelerated+healing+of+infected+wounds.">Wound dressing modifications for accelerated healing of infected wounds.</a> Int J Mol Sci. 24 (8), 7193 (2023).</li><li>Zeng, Q., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Wound+dressing:+From+nanomaterials+to+diagnostic+dressings+and+healing+evaluations.">Wound dressing: From nanomaterials to diagnostic dressings and healing evaluations.</a> ACS Nano. 16 (2), 1708-1733 (2022).</li><li>Singh, R., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Silver+sulfadiazine+loaded+core-shell+airbrushed+nanofibers+for+burn+wound+healing+application.">Silver sulfadiazine loaded core-shell airbrushed nanofibers for burn wound healing application.</a> Int J Pharm. 613, 121358 (2022).</li><li>Abdullahzadeh, M., Shafiee, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=To+compare+the+effect+of+sea+buckthorn+and+silver+sulfadiazine+dressing+on+period+of+wound+healing+in+patients+with+second-degree+burns:+A+randomized+triple-blind+clinical+trial.">To compare the effect of sea buckthorn and silver sulfadiazine dressing on period of wound healing in patients with second-degree burns: A randomized triple-blind clinical trial.</a> Wound Repair Regen. 29 (5), 732-740 (2021).</li><li>He, C., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Harnessing+biocompatible+nanofibers+and+silver+nanoparticles+for+wound+healing:+Sandwich+wound+dressing+versus+commercial+silver+sulfadiazine+dressing.">Harnessing biocompatible nanofibers and silver nanoparticles for wound healing: Sandwich wound dressing versus commercial silver sulfadiazine dressing.</a> Mater Sci Eng C Mater Biol Appl. 128, 112342 (2021).</li><li>Wu, J. -. J., Zhang, F., Liu, J., Yao, H. -. J., Wang, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+silver-containing+hydrofiber+dressing+on+burn+wound+healing:+A+meta-analysis+and+systematic+review.">Effect of silver-containing hydrofiber dressing on burn wound healing: A meta-analysis and systematic review.</a> J Cosmet Dermatol. 22 (5), 1685-1691 (2023).</li><li>Zhou, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thermosensitive+black+phosphorus+hydrogel+loaded+with+silver+sulfadiazine+promotes+skin+wound+healing.">Thermosensitive black phosphorus hydrogel loaded with silver sulfadiazine promotes skin wound healing.</a> J Nanobiotechnology. 21 (1), 330 (2023).</li><li>Da Silva, G. L. P., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Antimicrobial+dressing+of+silver+sulfadiazine-loaded+halloysite/cassava+starch-based+(bio)nanocomposites.">Antimicrobial dressing of silver sulfadiazine-loaded halloysite/cassava starch-based (bio)nanocomposites.</a> J Biomater Appl. 35 (9), 1096-1108 (2021).</li><li>De Francesco, F., Riccio, M., Jimi, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Contribution+of+topical+agents+such+as+hyaluronic+acid+and+silver+sulfadiazine+to+wound+healing+and+management+of+bacterial+biofilm.">Contribution of topical agents such as hyaluronic acid and silver sulfadiazine to wound healing and management of bacterial biofilm.</a> Medicina (Kaunas). 58 (6), 835 (2022).</li><li>Lee, J. S., Mallitt, K., Fischer, G., Saunderson, R. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+individual+patient+data+meta-analysis+of+wound+care+in+patients+with+toxic+epidermal+necrolysis.">An individual patient data meta-analysis of wound care in patients with toxic epidermal necrolysis.</a> Australas J Dermatol. 65 (2), 128-142 (2024).</li><li>Hsu, Y. -. H., Lin, Y. -. F., Chen, C. -. H., Chiu, Y. -. J., Chiu, H. -. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Far+infrared+promotes+wound+healing+through+activation+of+Notch1+signaling.">Far infrared promotes wound healing through activation of Notch1 signaling.</a> J Mol Med (Berl). 95 (11), 1203-1213 (2017).</li><li>Chai, L., Liu, Z. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Generalized+bullous+fixed+drug+eruption.">Generalized bullous fixed drug eruption.</a> Dermatitis. 34 (5), 364-365 (2023).</li><li>Surowiecka, A., Bara&#324;ska-Rybak, W., Stru&#380;yna, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Multidisciplinary+treatment+in+toxic+epidermal+necrolysis.">Multidisciplinary treatment in toxic epidermal necrolysis.</a> Int J Environ Res Public Health. 20 (3), 2217 (2023).</li><li>Staiger, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Comfrey:+a+clinical+overview.">Comfrey: a clinical overview.</a> Phytother Res. 26 (10), 1441-1448 (2012).</li><li>Shou, X. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Efficacy+of+chlorhexidine+and+povidone+iodine+in+preventing+surgical+site+infeceions.">Efficacy of chlorhexidine and povidone iodine in preventing surgical site infeceions.</a> Chinese Journal of Nosocomiology. 28 (17), 2702-2704 (2018).</li><li>Wen, J., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Advances+in+far-infrared+research:+therapeutic+mechanisms+of+disease+and+application+in+cancer+detection.">Advances in far-infrared research: therapeutic mechanisms of disease and application in cancer detection.</a> Lasers Med Sci. 39 (1), 41 (2024).</li><li>Li, K., Liu, N. -. F., Zhang, Y. -. X. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Therapeutic+effects+of+far-infrared+ray+in+treating+chronic+lower+extremity+lymphedema+with+dermatolymphangioadenitis.">Therapeutic effects of far-infrared ray in treating chronic lower extremity lymphedema with dermatolymphangioadenitis.</a> Journal of Shanghai Jiaotong University (Medical Science). 38 (09), 1060-1065 (2018).</li><li>Trommel, N., Hofland, H. W., van Komen, R. S., Dokter, J., van Baar, M. E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nursing+problems+in+patients+with+toxic+epidermal+necrolysis+and+Stevens-Johnson+syndrome+in+a+Dutch+burn+centre:+A+30-year+retrospective+study.">Nursing problems in patients with toxic epidermal necrolysis and Stevens-Johnson syndrome in a Dutch burn centre: A 30-year retrospective study.</a> Burns. 45 (7), 1625-1633 (2019).</li><li>Neema, S., Chatterjee, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Nano-silver+dressing+in+toxic+epidermal+necrolysis.">Nano-silver dressing in toxic epidermal necrolysis.</a> Indian J Dermatol Venereol Leprol. 83 (1), 121-124 (2017).</li></ol>

<table><tbody><tr><td>0.9% sodium chloride</td><td>SOUTHWEST PHARMACEUTICAL Co.,Ltd.</td><td></td><td></td></tr><tr><td>Bactroban ointment</td><td>Tianjin Shike Pharmaceutial Co.,Ltd.</td><td></td><td></td></tr><tr><td>Disposable Medical Kit (a dressing change box with sterile scissors)</td><td>Zhende Medical Co.,Ltd.</td><td>GB/T HYA-15</td><td></td></tr><tr><td>Fu fang Comfrey oil</td><td>Jian Min Ji Tuan Ye KaiTaiGuoYao Co.,Ltd.</td><td>GBZ20044385</td><td></td></tr><tr><td>Medical&amp;nbsp; Swab</td><td>Zhende Medical Co.,Ltd.</td><td>6926515436841</td><td></td></tr><tr><td>petroleum jelly oil gauze</td><td>Henan PiaoAn Group Co.,Ltd.</td><td>GB20153140848</td><td></td></tr><tr><td>Povidone-iodine</td><td>SHANDOING LIERKANG TECHOLOGY Co.,Ltd.</td><td></td><td></td></tr><tr><td>rb-bFGF</td><td>ZHU HAi Yi SHENG&amp;nbsp; BIOLOGICAL MEDICAL Co.,Ltd.</td><td>GBS1098077</td><td></td></tr><tr><td>silver sulfadiazine lipid hydrocolloid dressings</td><td>LABORATOIRES URO</td><td>3546895089623</td><td></td></tr><tr><td>Single-Use Sterile Rubber Surgical Gloves (both sterile and film)</td><td>SHANDOING YU YUAN RBBER GLOVES Co.,LTD</td><td>GB/T 7543-2020</td><td></td></tr><tr><td>Sterile Dispensing Syringe for Single Use (empty needles)</td><td>SHANDON WEIGAO GROUP MEDICAL POLYER Co.,LTD</td><td>GB/T 20142140076</td><td></td></tr><tr><td>sterile gauze rolls</td><td>Zhende Medical Co.,Ltd.</td><td>6959385745812</td><td></td></tr></tbody></table>

a standardized procedure of dressing management for toxic epidermal necrolysis

הליך סטנדרטי של ניהול חבישה לנקרוליזה אפידרמיסית רעילה

Toxic epidermal necrolysis (TEN) is a severe cutaneous adverse drug reaction with high mortality rates, demanding specialized wound care to address ...

a-standardized-procedure-dressing-management-for-toxic-epidermal

Research

JoVE Journal

Medicine

A Standardized Procedure of Dressing Management for Toxic Epidermal Necrolysis

189 Views.  The First Affiliated Hospital of Chongqing Medical University. זהו פרוטוקול חבישה סטנדרטי לחולים עם נקרוליזה אפידרמיסית רעילה. פרוטוקול חזק זה משתמש בחבישות יוני כסף שנועדו להאיץ את הריפוי ולהקל על הכאב, ולמזער את האשפוז.

Video: הליך סטנדרטי של ניהול חבישה לנקרוליזה אפידרמיסית רעילה

Using Visual and Narrative Methods to Achieve Fair Process in Clinical Care

The Institute of Medicine has targeted patient-centeredness as an important area of quality improvement. A major dimension of patient-centeredness is respect for patient's values, preferences, and expressed needs. Yet specific approaches to gaining this understanding and translating it to quality care in the clinical setting are lacking. From a patient perspective quality is not a simple concept but is best understood in terms of five dimensions: technical outcomes; decision-making efficiency; amenities and convenience; information and emotional support; and overall patient satisfaction. Failure to consider quality from this five-pronged perspective results in a focus on medical outcomes, without considering the processes central to quality from the patient's perspective and vital to achieving good outcomes. In this paper, we argue for applying the concept of fair process in clinical settings. Fair process involves using a collaborative approach to exploring diagnostic issues and treatments with patients, explaining the rationale for decisions, setting expectations about roles and responsibilities, and implementing a core plan and ongoing evaluation. Fair process opens the door to bringing patient expertise into the clinical setting and the work of developing health care goals and strategies. This paper provides a step by step illustration of an innovative visual approach, called photovoice or photo-elicitation, to achieve fair process in clinical work with acquired brain injury survivors and others living with chronic health conditions. Applying this visual tool and methodology in the clinical setting will enhance patient-provider communication; engage patients as partners in identifying challenges, strengths, goals, and strategies; and support evaluation of progress over time. Asking patients to bring visuals of their lives into the clinical interaction can help to illuminate gaps in clinical knowledge, forge better therapeutic relationships with patients living with chronic conditions such as brain injury, and identify patient-centered goals and possibilities for healing. The process illustrated here can be used by clinicians, (primary care physicians, rehabilitation therapists, neurologists, neuropsychologists, psychologists, and others) working with people living with chronic conditions such as acquired brain injury, mental illness, physical disabilities, HIV/AIDS, substance abuse, or post-traumatic stress, and by leaders of support groups for the types of patients described above and their family members or caregivers.

This paper illustrates an innovative visual approach (photovoice or photo-elicitation) to achieve fair process in clinical care for patients living with chronic health conditions, illuminate gaps in clinical knowledge, forge better therapeutic relationships, and identify patient-centered goals and possibilities for healing.

באמצעות שיטות חזותית הנרטיב להשגת הליך הוגן בטיפול קליני

The Institute of Medicine has targeted patient-centeredness as an important area of quality improvement. A major dimension of patient-centeredness is ...

Surgical Technique for Spinal Cord Delivery of Therapies: Demonstration of Procedure in Gottingen Minipigs

This is a compact visual description of a combination of surgical technique and device for the delivery of (gene and cell) therapies into the spinal cord. While the technique is demonstrated in the animal, the procedure is FDA-approved and currently being used for stem cell transplantation into the spinal cords of patients with ALS. While the FDA has recognized proof-of-principle data on therapeutic efficacy in highly characterized rodent models, the use of large animals is considered critical for validating the combination of a surgical procedure, a device, and the safety of a final therapy for human use. The size, anatomy, and general vulnerability of the spine and spinal cord of the swine are recognized to better model the human. Moreover, the surgical process of exposing and manipulating the spinal cord as well as closing the wound in the pig is virtually indistinguishable from the human. We believe that the healthy pig model represents a critical first step in the study of procedural safety.

Short visual description of the surgical technique and device used for the delivery of (gene and cell) therapies into the spinal cord. The technique is demonstrated in the animal but is entirely translatable and currently being used for human application.

טכניקה כירורגית למשלוח חוט שדרה של טיפולים: הפגנה של הנוהל בגטינגן Minipigs

This is a  compact visual description of a combination of surgical technique and device  for the  delivery of (gene and cell) therapies into the spinal ...

Tibial Nerve Transection - A Standardized Model for Denervation-induced Skeletal Muscle Atrophy in Mice

The tibial nerve transection model is a well-tolerated, validated, and reproducible model of denervation-induced skeletal muscle atrophy in rodents. Although originally developed and used extensively in the rat due to its larger size, the tibial nerve in mice is big enough that it can be easily manipulated with either crush or transection, leaving the peroneal and sural nerve branches of the sciatic nerve intact and thereby preserving their target muscles. Thus, this model offers the advantages of inducing less morbidity and impediment of ambulation than the sciatic nerve transection model and also allows investigators to study the physiologic, cellular and molecular biologic mechanisms regulating the process of muscle atrophy in genetically engineered mice. The tibial nerve supplies the gastrocnemius, soleus and plantaris muscles, so its transection permits the study of denervated skeletal muscle composed of fast twitch type II fibers and/or slow twitch type I fibers. Here we demonstrate the tibial nerve transection model in the C57Black6 mouse. We assess the atrophy of the gastrocnemius muscle, as a representative muscle, at 1, 2, and 4 weeks post-denervation by measuring muscle weights and fiber type specific cross-sectional area on paraffin-embedded histologic sections immunostained for fast twitch myosin.

The tibial nerve transection model is a well-tolerated, validated, and reproducible model of skeletal muscle atrophy. The model surgical protocol is described and demonstrated in C57Black6 mice. &#160;

חיתוך רוחב עצב הטיביאלי - מודל סטנדרטי לניוון שרירים שלד מושרה denervation בעכברים

The tibial nerve transection model is a well-tolerated, validated, and reproducible model of denervation-induced skeletal muscle atrophy in rodents. ...

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model

Fairness of skin, UV sensitivity and skin cancer risk all correlate with the physiologic function of the melanocortin 1 receptor, a Gs-coupled signaling protein found on the surface of melanocytes. Mc1r stimulates adenylyl cyclase and cAMP production which, in turn, up-regulates melanocytic production of melanin in the skin. In order to study the mechanisms by which Mc1r signaling protects the skin against UV injury, this study relies on a mouse model with "humanized skin" based on epidermal expression of stem cell factor (Scf). K14-Scf transgenic mice retain melanocytes in the epidermis and therefore have the ability to deposit melanin in the epidermis. In this animal model, wild type Mc1r status results in robust deposition of black eumelanin pigment and a UV-protected phenotype. In contrast, K14-Scf animals with defective Mc1r signaling ability exhibit a red/blonde pigmentation, very little eumelanin in the skin and a UV-sensitive phenotype. Reasoning that eumelanin deposition might be enhanced by topical agents that mimic Mc1r signaling, we found that direct application of forskolin extract to the skin of Mc1r-defective fair-skinned mice resulted in robust eumelanin induction and UV protection 1. Here we describe the method for preparing and applying a forskolin-containing natural root extract to K14-Scf fair-skinned mice and report a method for measuring UV sensitivity by determining minimal erythematous dose (MED). Using this animal model, it is possible to study how epidermal cAMP induction and melanization of the skin affect physiologic responses to UV exposure.

Epidermal melanin is induced by topical application of forskolin in a murine model of the fair-skinned UV-sensitive human. Pharmacologic manipulation of cAMP levels in the skin and epidermal darkening strongly protect against UV-mediated inflammation (sunburn) as measured by the minimum erythematous dose (MED) assay.

אינדוקציה תרופתי של עוריות מלנין והגנה מפני כוויות שמש בדגם מואנש מאוס

Fairness of skin, UV sensitivity and skin cancer  risk all correlate with the physiologic function of the melanocortin 1  receptor, a Gs-coupled signaling ...

A Standardized Obstacle Course for Assessment of Visual Function in Ultra Low Vision and Artificial Vision

We describe an indoor, portable, standardized course that can be used to evaluate obstacle avoidance in persons who have ultralow vision. Six sighted controls and 36 completely blind but otherwise healthy adult male (n=29) and female (n=13) subjects (age range 19-85 years), were enrolled in one of three studies involving testing of the BrainPort sensory substitution device. Subjects were asked to navigate the course prior to, and after, BrainPort training. They completed a total of 837 course runs in two different locations. Means and standard deviations were calculated across control types, courses, lights, and visits. We used a linear mixed effects model to compare different categories in the PPWS (percent preferred walking speed) and error percent data to show that the course iterations were properly designed. The course is relatively inexpensive, simple to administer, and has been shown to be a feasible way to test mobility function. Data analysis demonstrates that for the outcome of percent error as well as for percentage preferred walking speed, that each of the three courses is different, and that within each level, each of the three iterations are equal. This allows for randomization of the courses during administration.
Abbreviations: 
preferred walking speed (PWS) 
course speed (CS) 
percentage preferred walking speed (PPWS)

We describe an indoor, portable, standardized course that can be used to evaluate obstacle avoidance in persons who have ultralow vision. The course is relatively inexpensive, simple to administer, and has been shown to be reliable and reproducible.

מסלול מכשולים סטנדרטי להערכת תפקוד החזותי בUltra Low חזון וראייה מלאכותית

We describe an indoor, portable, standardized course that can be used to evaluate obstacle avoidance in persons who have ultralow vision. Six sighted ...

Therapeutic Effectiveness of a Dietary Supplement for Management of Halitosis in Dogs

Halitosis is a common complaint involving social and communicational problems in humans and also affects the pet-owner relationship. In this randomized placebo-controlled crossover clinical evaluation, we assessed&#160;the effectiveness of a dedicated dietary supplement to improve chronic halitosis in 32 dogs of different breeds and ages. This protocol describes how to evalute the presence of oral volatile suphur compunds, e.g. methyl mercaptan, hydrogen sulfide and dimethyl sulfide, by means of a portable gas chromatograph device coupled with a syringe, which was used to collect the breath, and a dedicated software, which allows the operator to monitor each compound concentration during each measurement, in a relatively short time (8 min).
A significant modification of halitosis parameters was observed after 30 days since the beginning of treatment (p &#60;0.05), while a long-lasting effect was still observed even 20 days after the suspension of the treatment. Portable gas chromatograph, which is also widely used in clinical practice, can be therefore used to confirm&#160;and control halitosis in humans and animals. Even though human and animal species present some differences,&#160;this innovative and alternative therapy for halitosis management might be extended to human clinical practice as an adjuvant dietary approach.

We describe a simple approach for diagnosis of halitosis in dogs as well as a dietary approach for its management. This protocol may be extended to the management of halitosis in humans in the near future.

יעילות טיפולית של תוסף תזונה לניהול של באשת בכלבים

Halitosis is a common complaint involving social and communicational problems in humans and also affects the pet-owner relationship. In this randomized ...

Standardized Measurement of Nasal Membrane Transepithelial Potential Difference (NPD)

We describe a standardized measurement of nasal potential difference (NPD). In this technique, cystic fibrosis transmembrane conductance regulator (CFTR) and the epithelial sodium channel (ENaC) function are monitored by the change in voltage across the nasal epithelium after the superfusion of solutions that modify ion channel activity. This is enabled by the measurement of the potential difference between the subcutaneous compartment and the airway epithelium in the nostril, utilizing a catheter in contact with the inferior nasal turbinate.
The test allows the measurement of the stable baseline voltage and the successive net voltage changes after perfusion of 100 &#181;M amiloride, an inhibitor of Na+ reabsorption in Ringer&#39;s solution; a chloride-free solution containing amiloride to drive chloride secretion and 10 &#181;M isoproterenol in a chloride-free solution with amiloride to stimulate the cyclic adenosine monophosphate (cAMP)-dependent chloride conductance related to CFTR.
This technique has the advantage of demonstrating the electrophysiological properties of two key components establishing the hydration of the airway surface liquid of the respiratory epithelium, ENaC, and CFTR. Therefore, it is a useful research tool for phase 2 and proof of concept trials of agents that target CFTR and ENaC activity for the treatment of cystic fibrosis (CF) lung disease. It is also a key follow-up procedure to establish CFTR dysfunction when genetic testing and sweat testing are equivocal. Unlike sweat chloride, the test is relatively more time consuming and costly. It also requires operator training and expertise to conduct the test effectively. Inter- and intra-subject variability has been reported in this technique especially in young or uncooperative subjects. To assist with this concern, interpretation has been improved through a recently validated algorithm.

Standardized Measurement of Nasal Membrane Transepithelial Potential Difference NPD

Here, we present a standardized protocol to measure the nasal potential difference (NPD). Cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC) function are evaluated by the change in the voltage across the nasal epithelium after superfusion of solutions that modify ion channel activity, providing an outcome measure.

מדידה מתוקננת של ההבדל Transepithelial פוטנציאל ממברנה האף (NPD)

We describe a standardized measurement of nasal potential difference (NPD). In this technique, cystic fibrosis transmembrane conductance regulator (CFTR) ...

A Minimally Invasive Model to Analyze Endochondral Fracture Healing in Mice Under Standardized Biomechanical Conditions

Bone healing models are necessary to analyze the complex mechanisms of fracture healing to improve clinical fracture treatment. During the last decade, an increased use of mouse models in orthopedic research was noted, most probably because mouse models offer a large number of genetically-modified strains and special antibodies for the analysis of molecular mechanisms of fracture healing. To control the biomechanical conditions, well-characterized osteosynthesis techniques are mandatory, also in mice. Here, we report on the design and use of a closed bone healing model to stabilize femur fractures in mice. The intramedullary screw, made of medical-grade stainless steel, provides through fracture compression an axial and rotational stability compared to the mostly used simple intramedullary pins, which show a complete lack of axial and rotational stability. The stability achieved by the intramedullary screw allows the analysis of endochondral healing. A large amount of callus tissue, received after stabilization with the screw, offers ideal conditions to harvest tissue for biochemical and molecular analyses. A further advantage of the use of the screw is the fact that the screw can be inserted into the femur with a minimally invasive technique without inducing damage to the soft tissue. In conclusion, the screw is a unique implant that can ideally be used in closed fracture healing models offering standardized biomechanical conditions.

This protocol describes a minimally invasive osteosynthesis technique using an intramedullary screw for standardized stabilization of femur fractures, which can be used to analyze endochondral bone healing in mice.

מודל פולשנית לניתוח שבר Endochondral ריפוי בעכברים בתנאים מתוקננים ביו-מכני

Bone healing models are necessary to analyze the complex mechanisms of fracture healing to improve clinical fracture treatment. During the last decade, an ...

Severe Burn Injury in a Swine Model for Clinical Dressing Assessment

Wound healing is a dynamic repair process and is the most complex biological process in human life. In response to burn injury, alterations in biological pathways impair the inflammation response, resulting in delayed wound healing. Impaired wound healing frequently occurs in patients with diabetes leading to unfavorable outcomes such as amputation. Hence, dressings having beneficial effect in promoting burn wound repair are needed. However, studies on burn wound treatment are limited due to lack of proper animal models. Our previous study demonstrated wound-healing performance in rat and swine models using a minimally invasive surgical technique. This study aimed to demonstrate a swine model of severe burn injury that eliminates wound contraction and more closely approximates the human processes of re-epithelialization and new tissue formation. This protocol provides a detailed procedure for creating consistent burn wounds and examining the wound-healing performance under the treatment of an experimental dressing in a swine model. Six burn wounds were created symmetrically on the dorsum, which were covered with a clinical dressing composed of four layers: an inner contact layer of experimental materials, an inner intermediate layer of waterproof film, an outer intermediate layer of gauze, and an outer layer of adhesive plaster. Upon the completion of experiments, wound closure, wound area, and Vancouver Scar Scale score were examined. The samples of skin resected from each animal post-sacrifice were histologically prepared and stained using hematoxylin and eosin staining. Antibacterial activity of each dressing in the context of wound healing was also examined. The application of the clinical dressing to the wounds in swine model mimics the biological processes of human wound healing with respect to the processes of epithelialization, cellular proliferation, and angiogenesis. Therefore, this swine model provides an easy-to-learn, cost-effective, and robust method to assess the effect of clinical dressings in severe burn injury.

To closely mimic the mode of burn injuries requires the interplay between clinical observation and studies in animal models. In this study, a swine model of severe burn injury was established to assess an experimental dressing in physiological and pathophysiological settings.

כוויות חמורות פציעה במודל החזירים להערכה קלינית ההלבשה

Wound healing is a dynamic repair process and is the most complex biological process in human life. In response to burn injury, alterations in biological ...

Remote Laboratory Management: Respiratory Virus Diagnostics

An uptick in recent pandemics (Ebola, Zika, MERS, influenza, etc.) underlines the need for a more 'nimble,' coordinated response that addresses a multitude of issues ranging from transportation, access, facilities, equipment, and communication to provider training. To address this need, we have developed an innovative, scalable, logistics-enhanced, mobile, laboratory facility for emergencies and epidemics in resource-constrained global settings. Utilizing a background in clinical operations as an academic medical center, we designed a rapidly-deployable, modular BSL-2 and BSL-3 facility with user-friendly software for tracking and management of drugs and supplies in remote regions during epidemics and outbreaks. Here, we present our intermodal, mobile, expandable shipping-container laboratory units. The design of the laboratory facilitates off-grid usage by minimizing power consumption and allowing alternate water sources. The unit's information communication technology (ICT) platform provides (i) user-friendly tablet-based documentation, (ii) enhanced tracking of patients and supplies, and (iii) integrated communication onsite with built-in telehealth capabilities. To ensure quality in remote environments, we have developed a checklist for a basic laboratory workflow and a protocol for respiratory viral diagnosis using reverse-transcription polymerase chain reaction (RT-PCR). As described, this innovative and comprehensive approach allows for the provision of laboratory capability in resource-limited global environments.

A rapidly-deployable, off-grid laboratory has been designed and built for remote, resource-constrained global settings. The features and critical aspects of the logistically-enhanced, expandable, multifunctional laboratory modules are explored. A checklist for a basic laboratory workflow and a protocol for a respiratory viral diagnostic test are developed and presented.