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University of Saskatchewan

7 ARTICLES PUBLISHED IN JoVE

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Neuroscience

The CApillary FEeder Assay Measures Food Intake in Drosophila melanogaster
Soeren Diegelmann 1, Annika Jansen 1, Shreyas Jois 1,2, Katharina Kastenholz 1, Laura Velo Escarcena 1, Nicole Strudthoff 1, Henrike Scholz 1
1Institute of Zoology, Albertus-Magnus University of Cologne, 2Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan

The CApillary FEeder (CAFE) assay is a simple, budget-friendly, highly reliable method for investigating mechanisms underlying food intake. Used with the highly versatile genetic model organism Drosophila melanogaster, it provides a powerful means of gaining new insights into regulatory mechanisms of food intake.

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Environment

Composition and Properties of Aquafaba: Water Recovered from Commercially Canned Chickpeas
Youn Young Shim 1,2,3, Rana Mustafa 1, Jianheng Shen 1, Kornsulee Ratanapariyanuch 1, Martin J. T. Reaney 1,2,3
1Department of Plant Sciences, University of Saskatchewan, 2Prairie Tide Chemicals Inc., 3Guangdong Saskatchewan Oilseed Joint Laboratory (GUSTO), Department of Food Science and Engineering, Jinan University

Aquafaba is a viscous juice from canned chickpea that, when stirred vigorously, produces a relatively stable white froth or foam. The primary research goal is to identify the components of aquafaba that contribute viscosifying/thickening properties using nuclear magnetic resonance (NMR), ultrafiltration, electrophoresis, and peptide mass fingerprinting.

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Developmental Biology

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
Kunal Baxi 1, Carlos E. de Carvalho 1
1Department of Biology, University of Saskatchewan

A step-by-step guide to probe loss of lysosomal acidity in the intestine of C. elegans using the pH-sensitive vital dye 5(6)-carboxy-2',7'-dichlorofluorescein diacetate (cDCFDA)

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Education

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase
John R. Miecznikowski 1, Jerry P. Jasinski 2, Manpreet Kaur 2, Sheila C. Bonitatibus 1, Emilse M. Almanza 1, Rami M. Kharbouch 1, Samantha E. Zygmont 1, Kendra R. Landy 1
1Department of Chemistry and Biochemistry, Fairfield University, 2Department of Chemistry, Keene State College

The preparation of SNS pincer cobalt(II) model complexes of liver alcohol dehydrogenase is presented here. The complexes can be prepared by reacting the ligand precursor with CoCl2·6H2O and can then be recrystallized by allowing diethyl ether to slowly diffuse into an acetonitrile solution that contains the cobalt complex.

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Genetics

Discovering CsgD Regulatory Targets in Salmonella Biofilm Using Chromatin Immunoprecipitation and High-Throughput Sequencing (ChIP-seq)
Melissa B. Palmer 1,2, Yejun Wang 3, Aaron P. White 1,2
1Vaccine and Infectious Disease Organization-International Vaccine Centre, 2Department of Biochemistry, Microbiology and Immunology, University of Saskatchewan, 3Department of Cell Biology and Genetics, Shenzhen University Health Science Center

Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) is a method used to establish interactions between transcription factors and the genomic sequences they control. This protocol outlines techniques for performing ChIP-seq with bacterial biofilms, using Salmonella enterica serovar Typhimurium bacterial biofilm as an example.

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Immunology and Infection

Visualizing Lung Cellular Adaptations during Combined Ozone and LPS Induced Murine Acute Lung Injury
Jessica Andrea Brocos Duda 1, Manpreet Kaur 1, Gurpreet K. Aulakh 1
1Small Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan

Combined ozone and bacterial endotoxin exposed mice show wide-spread cell death, including that of neutrophils. We observed cellular adaptations such as disruption of cytoskeletal lamellipodia, increased cellular expression of complex V ATP synthase subunit β and angiostatin in broncho-alveolar lavage, suppression of the lung immune response and delayed neutrophil recruitment.

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Neuroscience

Generating and Co-culturing Murine Primary Microglia and Cortical Neurons
Jian Park *1, Ruoqi Yu *1, Yifei Dong 1
1Department of Biochemistry, Microbiology & Immunology, University of Saskatchewan

This protocol describes a microglia-neuronal co-culture established from primary neuronal cells isolated from mouse embryos at embryonic days 15-16 and primary microglia generated from the brains of neonatal mice at post-natal days 1-2.

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