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Methods Article

Preparation of SNS Cobalt(II) Pincer Model Complexes of Liver Alcohol Dehydrogenase

6.9K Views

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06:31 min

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March 19th, 2020

DOI :

10.3791/60668-v

March 19th, 2020

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John R. Miecznikowski¹, Jerry P. Jasinski², Manpreet Kaur², Sheila C. Bonitatibus¹, Emilse M. Almanza¹, Rami M. Kharbouch¹, Samantha E. Zygmont¹, Kendra R. Landy¹

¹Department of Chemistry and Biochemistry, Fairfield University, ²Department of Chemistry, Keene State College

Summary

The preparation of SNS pincer cobalt(II) model complexes of liver alcohol dehydrogenase is presented here. The complexes can be prepared by reacting the ligand precursor with CoCl₂·6H₂O and can then be recrystallized by allowing diethyl ether to slowly diffuse into an acetonitrile solution that contains the cobalt complex.

Explore More Videos

SNS Cobalt II Pincer

Liver Alcohol Dehydrogenase

Single Crystals

Coordination Compounds

Cobalt II Chloride Hexahydrate

Recrystallization

Solute Dissolution

Slow Vapor Diffusion

Chemical Reaction

Chapters in this video

0:04

Introduction

0:52

Chloro-(n³-S,S,N)-[2,6-bis(N-isopropyl-N -methyleneimidazole-2-thione)pyridine]cobalt(II)tetrachlorocobaltate [4] Synthesis

1:42

Chloro-(n³-S,S,N)-[2,6-bis(N-isopropyl-N -methyleneimidazole-2-thione)pyridine]cobalt(II)tetrachlorocobaltate [4] Recrystallization

2:34

Chloro-(n³-S,S,N)-[2,6-bis(N-isopropyl-N -methylenetriazole-2-thione)pyridine]cobalt(II)tetrachlorocobaltate [5] Synthesis

3:22

Chloro-(n³-S,S,N)-[2,6-bis(N-isopropyl-N'-methylenetriazole-2-thione)pyridine]cobalt(II) tetrachlorocobaltate [5] Recrystallization

4:28

Results: Representative Liver Alcohol Dehydrogenase Complex Analyses

5:41

Conclusion

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