Generating and Co-culturing Murine Primary Microglia and Cortical Neurons

1.4K Views

08:47 min

July 26th, 2024

DOI :

10.3791/67078-v

July 26th, 2024


Transcript

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Microglia

Chapters in this video

0:00

Introduction

1:15

Brain Isolation from Neonatal Mice for Primary Microglia Culture

2:19

Preparing and Maintaining Mixed Glia Culture Derived from Neonatal Mice Brains

3:45

Isolating Brains from E16‐E18 Mouse Embryos for Primary Neuron Culture

4:57

Brain Dissociation and Seeding of Cortical Neurons in Cell Culture

6:53

Co‐Culture of Primary Neurons and Microglia for Studying Microglia‐Neuron Interactions

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