Rapid fluctuations in extracellular dopamine (DA) mediate both reward processing and motivated behavior in mammals. This manuscript describes the combined use of fast scan cyclic voltammetry (FSCV) and intra-oral tastant administration to determine how tastants alter rapid dopamine release in awake, freely moving rats.
The goal of this protocol is to directly manipulate ventral tegmental area receptors to study their contribution to subsecond dopamine release.
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